製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DDX3Y
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment, corresponding to a sequence within amino acids 70-316 of Human DDX3Y.

  • ポジティブ・コントロール
    • 293T, A431, H1299, HeLa, HepG2, Molt4, and Raji cells and whole cell lysates.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 73 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能
    Probable ATP-dependent RNA helicase. May play a role in spermatogenesis.
  • 組織特異性
    Testis-specific. Expressed predominantly in spermatogonia.
  • 関連疾患
    Note=DDX3Y is located in the 'azoospermia factor a' (AZFa) region on chromosome Y which is deleted in Sertoli cell-only syndrome. This is an infertility disorder in which no germ cells are visible in seminiferous tubules leading to azoospermia.
  • 配列類似性
    Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 細胞内局在
    Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm in an XPO1-dependent manner.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATP dependent RNA helicase DDX3Y antibody
    • ATP-dependent RNA helicase DDX3Y antibody
    • DBY antibody
    • DDX3Y antibody
    • DDX3Y_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 3 Y linked antibody
    • DEAD box protein 3 Y chromosomal antibody
    • DEAD box protein 3 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide Y chromosome antibody
    • Y-chromosomal antibody
    see all

画像

  • All lanes : Anti-DDX3Y antibody (ab95979) at 1/10000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 73 kDa
  • ab95979 at 1/200 dilution staining DDX3Y in HeLa cells by Immunofluorescence, Paraformaldehyde-fixed. Lower image is merged with DNA probe.

参考文献

ab95979 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us.

The lab has confirmed that the immunogen for ab95979 shares 96% identity with DDX3X, so it has high chance of cross-reacting with DDX3X which is why it is cross reacting with Hela cells.

I hope this inform...

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One possible reason of this cross reactivity is due to the sequence homology between DDX3Y and DDX3X. Please click the link below to see the DDX3Y sequence alignment with DDX3X; the similarity is 92% which is more ...

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I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future or...

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Thank you for contacting Abcam regarding ab95979.


I am sorry that the customer has experienced difficulties with this antibody in IF. I reviewed the protocol details provided and I would just like to clarify if the customer is using a h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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