Anti-DDX27 抗体 [2251C2a] (ab50696)
Key features and details
- Mouse monoclonal [2251C2a] to DDX27
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-DDX27 antibody [2251C2a]
DDX27 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2251C2a] to DDX27 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant human DDX27
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ポジティブ・コントロール
- HeLa cells and HeLa and NIH3T3 cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
ab50696 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane. -
ポリ/モノ
モノクローナル -
クローン名
2251C2a -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50696の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/50. Predicted molecular weight: 90 kDa.
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ICC |
1/5.
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特記事項 |
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WB
1/50. Predicted molecular weight: 90 kDa. |
ICC
1/5. |
ターゲット情報
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機能
Probable ATP-dependent RNA helicase. -
配列類似性
Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 55661 Human
- Entrez Gene: 228889 Mouse
- SwissProt: Q96GQ7 Human
- SwissProt: Q921N6 Mouse
- Unigene: 404263 Human
- Unigene: 65234 Human
- Unigene: 295031 Mouse
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別名
- C86129 antibody
- cb843 antibody
- DDX27 antibody
see all
画像
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Anti-DDX27 antibody [2251C2a] (ab50696) at 1/50 dilution + HeLa whole cell lysate at 25 µg
Secondary
Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 90 kDa
Observed band size: 90 kDa -
Anti-DDX27 antibody [2251C2a] (ab50696) at 1/50 dilution + NIH3T3 whole cell lyaste at 25 µg
Secondary
Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 90 kDa
Observed band size: 90 kDa -
ab50696 at 1/5 dilution staining human DDX27 in HeLa cells by Immunocytochemistry. A 1/200 dilution of AlexaFluor488 Goat Anti-mouse IgG was used as secondary.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab50696 は論文での使用が確認できていません。