製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DDX19B
  • 特異性
    The immunogen sequence is highly conserved with respect to DDX19A (GeneID 55308), but crossreactivity of the antibody with this homolog has not been assessed.
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Guinea pig, Cow, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis, Other
  • 免疫原

    Synthetic peptide corresponding to a region between C terminal residues 450 and 479 of Human DDX19B. (NP_009173.1)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa , 293T and NIH3T3 cells

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab70207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    ATP-dependent RNA helicase involved in mRNA export from the nucleus.
  • 配列類似性
    Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 細胞内局在
    Cytoplasm. Nucleus > nuclear pore complex. Nucleus membrane. Nuclear pore complex cytoplasmic fibrils.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATP-dependent RNA helicase DDX19B antibody
    • DBP5 antibody
    • Dbp5, yeast, homolog of antibody
    • DD19B_HUMAN antibody
    • DDX19 antibody
    • DDX19B antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B antibody
    • DEAD box protein 19B antibody
    • DEAD box RNA helicase DEAD5 antibody
    • DEAD-box protein 5 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog) antibody
    • DEAD/H BOX 19 antibody
    • helicase DEAD5 antibody
    • RNAh antibody
    • TDBP antibody
    • yeast Dbp5 homolog antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX19B with ab70207 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-DDX19B antibody (ab70207) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds

参考文献

ab70207 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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