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A region within synthetic peptide: CDRRLRGVKD GGRRDSASYR DRSETDRAGY ANGSGYGSPN SAFGAQAGQ Y, corresponding to C terminal amino acids 505-554 of Human DDX17
Our Abpromise guarantee covers the use of ab66764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay 1:62500.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 60, 65, 66 kDa (predicted molecular weight: 72 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling DDX17 with ab66764 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubules. Magnification: 400X.