製品の概要

  • 製品名Anti-DDOST antibody
    DDOST 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DDOST
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    A region within synthetic peptide: NINVETISAF IDGGGSVLVA ASSDIGDPLR ELGSECGIEF DEEKTAVIDH, corresponding to N terminal amino acids 110-159 of Human DDOST

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab65533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

ターゲット情報

  • 関連性DDOST (Dolichyl-diphosphooligosaccharide-protein glycosyltransferase) is a component of the oligosaccharyltransferase complex. This complex catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum and co-purifies with ribosomes. DDOST is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.
  • 細胞内局在Endoplasmic reticulum membrane; Single-pass type I membrane protein.
  • 参照データベース
  • 別名
    • Advanced glycation endproduct receptor 1 antibody
    • AGE R1 antibody
    • AGER1 antibody
    • CDG1R antibody
    • DDOST 48 kDa subunit antibody
    • Dolichyl diphosphooligosaccharide protein glycosyltransferase subunit (non catalytic) antibody
    • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit antibody
    • Dolichyl-diphosphooligosaccharide-protein glycosyltransferase antibody
    • EC 2.4.1.119 antibody
    • KIAA0115 antibody
    • MGC2191 antibody
    • OK/SW-cl.45 antibody
    • OKSWcl45 antibody
    • Oligosaccharyl transferase 48 kDa subunit antibody
    • Oligosaccharyltransferase 48 kDa subunit antibody
    • Oligosaccharyltransferase antibody
    • Oligosaccharyltransferase subunit 48 antibody
    • OST antibody
    • OST48 antibody
    • RP23-25C1.4 antibody
    • WBP1 antibody
    • wu:fa11d01 antibody
    • zgc:66068 antibody
    • zgc:77478 antibody
    see all

Anti-DDOST antibody 画像

  • Anti-DDOST antibody (ab65533) at 5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa

Anti-DDOST antibody (ab65533) 使用論文

ab65533 has not yet been referenced specifically in any publications.

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