製品の概要

  • 製品名
    Anti-DDIT4L antibody
    DDIT4L 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DDIT4L
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to Human DDIT4L aa 51-100 (internal sequence).
    Sequence:

    ESTCQNLVKMLENCLSKSKQTKLGCSKVLVPEKLTQRIAQDVLRLSSTEP

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • 293T cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性
    DDIT4L inhibits cell growth by regulating the FRAP1 pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. It is up regulated in atherosclerotic plaques relative to healthy segments of the same artery.
  • 細胞内局在
    Cytoplasmic
  • 参照データベース
    see all
  • 別名
    • DNA damage inducible transcript 4 like antibody
    • DNA damage inducible transcript 4 like protein antibody
    • HIF 1 responsive protein RTP801 like antibody
    • Homolog of mouse SMHS1 antibody
    • Protein regulated in development and DNA damage response 2 antibody
    • Protein regulated in development and DNA damage responses 2 antibody
    • REDD 2 antibody
    • REDD2 antibody
    • Regulated in development and DNA damage response 2 antibody
    • RTP801L antibody
    see all

画像

  • Western blot - Anti-DDIT4L antibody (ab84562)
    Western blot - Anti-DDIT4L antibody (ab84562)
    Anti-DDIT4L antibody (ab84562) at 1 µg/ml (5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa
    Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration: 12%

プロトコール

参考文献

This product has been referenced in:
  • Simonson B  et al. DDiT4L promotes autophagy and inhibits pathological cardiac hypertrophy in response to stress. Sci Signal 10:N/A (2017). WB ; Mouse . Read more (PubMed: 28246202) »

See 1 Publication for this product

ab84562 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

2 reviews or Q&A

10
Votes: Highest First

LOT NUMBER -- NOT SPECIFIED --
ORDER NUMBER -- NOT SPECIFIED --
DESCRIPTION OF THE PROBLEM No signal or weak signal and our blots dont reliably work with this antibody
SAMPLE Mouse cardiomyocyte protein extracts (cell cultured) Mouse hea...

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According to our records, ab84562 was proving difficult to use in WB with mouse samples and we were in contact in order to help resolve the issue.
Looking at our correspondence, it appears that we are awaiting to hear back in order to resolve this...

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LOT NUMBER -- NOT SPECIFIED --
ORDER NUMBER -- NOT SPECIFIED --
DESCRIPTION OF THE PROBLEM No signal or weak signal and our blots dont reliably work with this antibody
SAMPLE Mouse cardiomyocyte protein extracts (cell cultured) Mouse hea...

Read More

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.
I would like to point out that mouse samples have not been tried with this antibody, and we therefore do not know...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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