製品の概要

  • 製品名Anti-DDIT4 antibody
    DDIT4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DDIT4
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human DDIT4 aa 20-49 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 54541

  • ポジティブ・コントロール
    • Jurkat cell line lysates. Human lung carcinoma tissue.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab63059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
ELISA 1/1000.
IHC-P 1/50.

ターゲット情報

  • 機能Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death.
  • 組織特異性Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level).
  • 配列類似性Belongs to the DDIT4 family.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DDIT4 antibody
    • DDIT4_HUMAN antibody
    • Dig2 antibody
    • DNA damage inducible transcript 4 antibody
    • DNA damage inducible transcript 4 protein antibody
    • DNA damage-inducible transcript 4 protein antibody
    • FLJ20500 antibody
    • HIF 1 responsive protein RTP801 antibody
    • HIF 1 responsive RTP801 antibody
    • HIF-1 responsive protein RTP801 antibody
    • Protein regulated in development and DNA damage response 1 antibody
    • REDD-1 antibody
    • REDD1 antibody
    • RTP801 antibody
    see all

Anti-DDIT4 antibody 画像

  • Anti-DDIT4 antibody (ab63059) at 1/60 dilution + Jurkat cell line lysates at 35 µg

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • ab63059, at 1/50 dilution, staining DDIT4 in formalin-fixed, paraffin-embedded human lung carcinoma tissue by immunohistochemistry. ab63059 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
  • ab63059 staining DDIT4 in Human artery tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 60 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/20) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/250) was used as the secondary antibody.

    See Abreview

Anti-DDIT4 antibody (ab63059) 使用論文

This product has been referenced in:
  • Heller BA  et al. Functionally distinct PI 3-kinase pathways regulate myelination in the peripheral nervous system. J Cell Biol 204:1219-36 (2014). WB . Read more (PubMed: 24687281) »
  • Jin Z  et al. Decreased expression of histone deacetylase 10 predicts poor prognosis of gastric cancer patients. Int J Clin Exp Pathol 7:5872-9 (2014). IHC ; Human . Read more (PubMed: 25337229) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (artery)
Specification artery
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
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投稿 Mar 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"