ab63059, at 1/50 dilution, staining DDIT4 in formalin-fixed, paraffin-embedded human lung carcinoma tissue by immunohistochemistry. ab63059 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDIT4 antibody (ab63059)This image is courtesy of an anonymous Abreview
ab63059 staining DDIT4 in Human artery tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 60 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/20) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/250) was used as the secondary antibody.