製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DDIT4
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human DDIT4 aa 20-49 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 54541

  • ポジティブ・コントロール
    • Jurkat cell line lysates. Human lung carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
ELISA 1/1000.
IHC-P 1/50.

ターゲット情報

  • 機能
    Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death.
  • 組織特異性
    Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level).
  • 配列類似性
    Belongs to the DDIT4 family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DDIT4 antibody
    • DDIT4_HUMAN antibody
    • Dig2 antibody
    • DNA damage inducible transcript 4 antibody
    • DNA damage inducible transcript 4 protein antibody
    • DNA damage-inducible transcript 4 protein antibody
    • FLJ20500 antibody
    • HIF 1 responsive protein RTP801 antibody
    • HIF 1 responsive RTP801 antibody
    • HIF-1 responsive protein RTP801 antibody
    • Protein regulated in development and DNA damage response 1 antibody
    • REDD-1 antibody
    • REDD1 antibody
    • RTP801 antibody
    see all

Anti-DDIT4 antibody 画像

  • Anti-DDIT4 antibody (ab63059) at 1/60 dilution + Jurkat cell line lysates at 35 µg

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • ab63059, at 1/50 dilution, staining DDIT4 in formalin-fixed, paraffin-embedded human lung carcinoma tissue by immunohistochemistry. ab63059 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
  • ab63059 staining DDIT4 in Human artery tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 60 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/20) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/250) was used as the secondary antibody.

    See Abreview

Anti-DDIT4 antibody (ab63059) 使用論文

This product has been referenced in:
  • Heller BA  et al. Functionally distinct PI 3-kinase pathways regulate myelination in the peripheral nervous system. J Cell Biol 204:1219-36 (2014). WB . Read more (PubMed: 24687281) »
  • Jin Z  et al. Decreased expression of histone deacetylase 10 predicts poor prognosis of gastric cancer patients. Int J Clin Exp Pathol 7:5872-9 (2014). IHC ; Human . Read more (PubMed: 25337229) »

See all 5 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Bos taurus Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (4-15% Gradient)
Loading amount
20 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Julie Kim

Verified customer

投稿 May 04 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (artery)
Specification
artery
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Mar 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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