製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DDIT4
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human DDIT4.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HepG2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

ターゲット情報

  • 機能
    Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death.
  • 組織特異性
    Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level).
  • 配列類似性
    Belongs to the DDIT4 family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DDIT4 antibody
    • DDIT4_HUMAN antibody
    • Dig2 antibody
    • DNA damage inducible transcript 4 antibody
    • DNA damage inducible transcript 4 protein antibody
    • DNA damage-inducible transcript 4 protein antibody
    • FLJ20500 antibody
    • HIF 1 responsive protein RTP801 antibody
    • HIF 1 responsive RTP801 antibody
    • HIF-1 responsive protein RTP801 antibody
    • Protein regulated in development and DNA damage response 1 antibody
    • REDD-1 antibody
    • REDD1 antibody
    • RTP801 antibody
    see all

画像

  • Anti-DDIT4 antibody (ab124213) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

プロトコール

参考文献

ab124213 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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