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Our Abpromise guarantee covers the use of ab49763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 23497283|
|WB||1/100 - 1/1000.|
|ICC||1/100 - 1/1000.|
|EMSA||Use at an assay dependent concentration. PubMed: 22147708|
This image is courtesy of an anonymous Abreview
Sample 1, 2, 3, 4, 5, 6 are the lysates from the strains expressing the tagged protein.
5% milk blocking was used for 1 hour.
This image is courtesy of an anonymous AbreviewSaccharomyces cerevisiae purified Flag-tag protein was detected by using ab49763 (1/1000). Please refer to abreview for further experimental details.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"