Anti-DDDDK tag 抗体 [FG4R] - (Equivalent to FLAG antibodies from Sigma) (ab125243)

製品の概要

  • 製品名
    Anti-DDDDK tag antibody [FG4R] - (Equivalent to FLAG antibodies from Sigma)
    DDDDK tag 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [FG4R] to DDDDK tag - (Equivalent to FLAG antibodies from Sigma)
  • 特異性
    Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins.
  • アプリケーション
    適用あり: WB, ELISA, Dot blot, IP, In situ hybridizationmore details
  • 免疫原

    Synthetic peptide corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    DYKDDDDK

  • ポジティブ・コントロール
    • HEK293 cells transfected with DYKDDDDK
  • 特記事項
    If stored at 4 °C please add 0.1% sodium azide.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度
    Ascites
  • 特記事項(精製)
    ab125243 was purified by protein A affinity chromatography from mouse ascites fluid
  • ポリ/モノ
    モノクローナル
  • クローン名
    FG4R
  • アイソタイプ
    IgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab125243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/3000. (with ECL)
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
In situ hybridization 1/500 - 1/2000.

ターゲット情報

  • 関連性
    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • 別名
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

画像

  • All lanes : Anti-DDDDK tag antibody [FG4R] - (Equivalent to FLAG antibodies from Sigma) (ab125243) at 1/1000 dilution

    Lane 1 : HEK293 cell lysate non-transfected
    Lane 2 : HEK293 cell lysate transfected with DDDDK tagged protein vector

    Developed using the ECL technique

参考文献

This product has been referenced in:
  • Tang S  et al. RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase. Nat Commun 5:4657 (2014). WB . Read more (PubMed: 25118589) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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