Anti-DDDDK tag 抗体 - (Equivalent to FLAG antibodies from Sigma) (HRP) (ab2491)

製品の概要

  • 製品名
    Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (HRP)
    DDDDK tag 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to DDDDK tag - (Equivalent to FLAG antibodies from Sigma) (HRP)
  • 標識
    HRP
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 免疫原

    Peptide xxxDDDDK conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.

  • 特記事項
    Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    50 mM HEPES pH 7.0, 0.1 M NaCl, 0.2% BSA, 0.01% Thimerosal
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antibodies were affinity purified using the peptide coupled to a different carrier.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgY
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    The affinity purified antibodies were analyzed by ELISA and Western Blot analyses.
    In a direct ELISA against 1 ug of ECS peptide coupled to BSA as sample, conjugate diluted 1:35,000 produced an O.D.=1.0 in a 15 minute reaction using TMB as substrate.
    Optimal conjugate working dilutions should be determined for your particular assay conditions,
  • ターゲット情報

    • 関連性
      This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
    • 別名
      • DDDDK epitope tag antibody
      • DDDK antibody
      • ddk antibody
      • DYKDDDDK antibody
      • DYKDDDDK epitope tag antibody
      • DYKDDDDK tag antibody
      • ECS epitope tag antibody
      • ECS tag antibody
      • Enterokinase Cleavage Site epitope tag antibody
      • Enterokinase Cleavage Site tag antibody
      • FLAG antibody
      • FLAG tag antibody
      see all

    プロトコール

    参考文献

    This product has been referenced in:
    • Walsh CP  et al. Three-dimensional Structure of CaV3.1: COMPARISON WITH THE CARDIAC L-TYPE VOLTAGE-GATED CALCIUM CHANNEL MONOMER ARCHITECTURE. J Biol Chem 284:22310-21 (2009). WB . Read more (PubMed: 19520861) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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