製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 特記事項(精製)ECS (Enterokinase Cleavage Site) Affinity Purified.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab21536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 20371678
ELISA Use at an assay dependent concentration.
Use at a concentration of 2 - 10 µg/ml for coating or 0.03 - 1 µg/ml as a primary.
WB Use at an assay dependent concentration.
Use at a concentration of 0.1 - 1 µg/ml (colorimetric) or 0.03 - 1µg/ml (chemiluminescence).
ICC Use a concentration of 0.5 - 5 µg/ml.

ターゲット情報

  • 関連性This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • 別名
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Anti-DDDDK tag antibody 画像

  • ab21536 staining exogenous murine FLICE inhibitory protein (FLIP) tagged with FLAG from mouse thyroid tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with BSA prior to incubation with the antibody for 16 hours. A biotinylated goat polyclonal antibody was used as the secondary.

    See Abreview

Anti-DDDDK tag antibody (ab21536) 使用論文

This product has been referenced in:
  • Orr AG  et al. Astrocytic adenosine receptor A2A and Gs-coupled signaling regulate memory. Nat Neurosci 18:423-34 (2015). IP . Read more (PubMed: 25622143) »
  • Gerdes JM  et al. Ciliary dysfunction impairs beta-cell insulin secretion and promotes development of type 2 diabetes in rodents. Nat Commun 5:5308 (2014). Read more (PubMed: 25374274) »

See all 10 Publications for this product

Product Wall

Merci de nous avoir contactés.

Nous avons plusieurs anti-FLAG anticorps au catalogue par contre aucun n’a été testé en microscopie électronique.

Cependant, je peux vous proposer :

a...

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T)
Loading amount 20 µg
Specification HEK293T
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Ms. Silje Markussen

Verified customer

投稿 Aug 16 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (thyroid)
Specification thyroid
Fixative Formaldehyde
Antigen retrieval step None
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%
Username

Dr. Yujiang FANG

Verified customer

投稿 Mar 29 2007

Thank you for your enquiry. Jen is out of the office, so I am answering her correspondence. I understand that you used a third antibody successfully in this protocol, but each antibody is different and sometimes must be optimized to work well. W...

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Thank you for your enquiry. This antibody should recognize both N- terminus or C- terminus DDDDK tag. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"