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Peptide xxxDDDDK conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.
Our Abpromise guarantee covers the use of ab1162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 1 µg/ml.
Antibody was analyzed by western blot using various amino-terminal and carboxy-terminal DDDDK fusion proteins and Invitrogen Positope" R900-40. A 1/5000 dilution gave bands against 0.1, 1.0 and 10 ng of the fusion proteins and 100 ng and 25 ng of the Positope.
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||Use a concentration of 1 - 5 µg/ml.
As coating antibody in sELISA.
|IP||1/40. PubMed: 25419851|