製品の概要
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製品名Anti-DDB2 antibody [2246C4a]
DDB2 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [2246C4a] to DDB2
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由来種Mouse
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アプリケーション適用あり: IHC-P, ICC/IF, WB, Dot blot, Flow Cytmore details
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種交差性交差種: Human
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免疫原
Recombinant fragment (N-terminal) Human.
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ポジティブ･コントロール
- HeLa whole cell lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
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バッファーPreservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Protein G purified
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特記事項（精製）Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.
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ポリ/モノモノクローナル
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クローン名2246C4a
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アイソタイプIgG1
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研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab51017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | 1/50. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa). | |
| Dot blot | Use at an assay dependent concentration. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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機能Required for DNA repair. Binds to DDB1 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. Isoform D1 and isoform D2 inhibit UV-damaged DNA repair.
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組織特異性Ubiquitously expressed; with highest levels in corneal endothelium and lowest levels in brain. Isoform D1 is highly expressed in brain and heart. Isoform D2, isoform D3 and isoform D4 are weakly expressed.
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パスウェイProtein modification; protein ubiquitination.
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関連疾患Defects in DDB2 are a cause of xeroderma pigmentosum complementation group E (XP-E) [MIM:278740]; also known as xeroderma pigmentosum V (XP5). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities.
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配列類似性Belongs to the WD repeat DDB2/WDR76 family.
Contains 5 WD repeats. -
ドメインThe DWD box is required for interaction with DDB1.
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翻訳後修飾Phosphorylation by ABL1 negatively regulate UV-DDB activity.
Ubiquitinated by CUL4A in response to UV irradiation. Ubiquitination appears to both impair DNA-binding and promotes ubiquitin-dependent proteolysis. Degradation of DDB2 at sites of DNA damage may be a prerequisite for their recognition by XPC and subsequent repair. CUL4A-mediated degradation appears to be promoted by ABL1. -
細胞内局在Nucleus. Accumulates at sites of DNA damage following UV irradiation.
- Information by UniProt
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参照データベース
- Entrez Gene: 1643 Human
- Omim: 600811 Human
- SwissProt: Q92466 Human
- Unigene: 700338 Human
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別名
- damage-specific DNA binding protein 2 antibody
- Damage-specific DNA-binding protein 2 antibody
- DDB p48 subunit antibody
see all
画像
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![Western blot - Anti-DDB2 antibody [2246C4a] (ab51017)](http://a.abcam.com/ps/products/51/ab51017/Images/ddb2.jpg)
Western blot - Anti-DDB2 antibody [2246C4a] (ab51017)Anti-DDB2 antibody [2246C4a] (ab51017) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 48 kDa
Observed band size: 45 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB2 antibody [2246C4a] (ab51017)](http://a.abcam.com/ps/products/51/ab51017/Images/DDB2-Primary-antibodies-ab51017-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB2 antibody [2246C4a] (ab51017)IHC image of ab51017 staining in human normal cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Immunocytochemistry/ Immunofluorescence - Anti-DDB2 antibody [2246C4a] (ab51017)](http://a.abcam.com/ps/products/51/ab51017/Images/DDB2-Primary-antibodies-ab51017-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DDB2 antibody [2246C4a] (ab51017)ICC/IF image of ab51017 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51017, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Flow Cytometry - Anti-DDB2 antibody [2246C4a] (ab51017)](http://a.abcam.com/ps/products/51/ab51017/Images/DDB2-Primary-antibodies-ab51017-4.jpg)
Flow Cytometry - Anti-DDB2 antibody [2246C4a] (ab51017)Overlay histogram showing HeLa cells stained with ab51017 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51017, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
参考文献
This product has been referenced in:
- Zhu B et al. The protective role of DOT1L in UV-induced melanomagenesis. Nat Commun 9:259 (2018). Read more (PubMed: 29343685) »
- E X et al. A novel DDB2-ATM feedback loop regulates human cytomegalovirus replication. J Virol 88:2279-90 (2014). Human . Read more (PubMed: 24335308) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"