Anti-DDB1 抗体 [EPR6089] (ab109027)


  • 製品名Anti-DDB1 antibody [EPR6089]
    DDB1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6089] to DDB1
  • アプリケーション適用あり: ICC/IF, WB, IP, IHC-P, ICCmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues at the C-terminus of Human DDB1.

  • ポジティブ・コントロール
    • HepG2, HeLa, NIH3T3, and Human platelet lysates, Human breast tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: UV-treated HeLa
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-DDB1 antibody (HRP) [EPR6089] (ab199026)



Our Abpromise guarantee covers the use of ab109027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100.
WB 1/50000 - 1/200000. Detects a band of approximately 130 kDa (predicted molecular weight: 127 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.
    • パスウェイProtein modification; protein ubiquitination.
    • 配列類似性Belongs to the DDB1 family.
    • 翻訳後修飾Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.
    • 細胞内局在Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Damage specific DNA binding protein 1 antibody
      • Damage-specific DNA-binding protein 1 antibody
      • DDB 1 antibody
      • DDB p127 subunit antibody
      • Ddb1 antibody
      • DDB1_HUMAN antibody
      • DDBa antibody
      • DNA damage binding protein 1 antibody
      • DNA damage-binding protein 1 antibody
      • DNA damage-binding protein a antibody
      • HBV X-associated protein 1 antibody
      • UV damaged DNA binding factor antibody
      • UV damaged DNA binding protein 1 antibody
      • UV DDB 1 antibody
      • UV DDB1 antibody
      • UV-damaged DNA-binding factor antibody
      • UV-damaged DNA-binding protein 1 antibody
      • UV-DDB 1 antibody
      • X associated protein 1 antibody
      • XAP 1 antibody
      • XAP-1 antibody
      • XAP1 antibody
      • Xeroderma pigmentosum group E complementing protein antibody
      • Xeroderma pigmentosum group E-complementing protein antibody
      • XPCe antibody
      • XPE antibody
      • XPE BF antibody
      • XPE binding factor antibody
      • XPE-BF antibody
      • XPE-binding factor antibody
      see all

    Anti-DDB1 antibody [EPR6089] 画像

    • ab109027 stained UV-treated HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109027 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150081 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • All lanes : Anti-DDB1 antibody [EPR6089] (ab109027) at 1/50000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : NIH3T3 cell lysate
      Lane 4 : Human platelet lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 127 kDa
    • Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab109027 at a dilution of 1/100.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-DDB1 antibody [EPR6089] (ab109027) 使用論文

    ab109027 has not yet been referenced specifically in any publications.

    Product Wall

    Merci de votre intérêt pour ab21080 et merci pour m'avoir fourni la séquence de la DDB1 du xénope.

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