製品の概要

  • 製品名Anti-DDB1 antibody
    DDB1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DDB1
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Cow
  • 免疫原

    Recombinant fragment, corresponding to a sequence within amino acids 749-1123 of Human DDB1 (NP_001914).

  • ポジティブ・コントロール
    • 293T, A431, HeLa, HepG, MOLT4, Raji and NIH3T3 cells. Cal27 Xenograft tissue.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 127 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the DDB1 family.
  • 翻訳後修飾Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.
  • 細胞内局在Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Damage specific DNA binding protein 1 antibody
    • Damage-specific DNA-binding protein 1 antibody
    • DDB 1 antibody
    • DDB p127 subunit antibody
    • Ddb1 antibody
    • DDB1_HUMAN antibody
    • DDBa antibody
    • DNA damage binding protein 1 antibody
    • DNA damage-binding protein 1 antibody
    • DNA damage-binding protein a antibody
    • HBV X-associated protein 1 antibody
    • UV damaged DNA binding factor antibody
    • UV damaged DNA binding protein 1 antibody
    • UV DDB 1 antibody
    • UV DDB1 antibody
    • UV-damaged DNA-binding factor antibody
    • UV-damaged DNA-binding protein 1 antibody
    • UV-DDB 1 antibody
    • X associated protein 1 antibody
    • XAP 1 antibody
    • XAP-1 antibody
    • XAP1 antibody
    • Xeroderma pigmentosum group E complementing protein antibody
    • Xeroderma pigmentosum group E-complementing protein antibody
    • XPCe antibody
    • XPE antibody
    • XPE BF antibody
    • XPE binding factor antibody
    • XPE-BF antibody
    • XPE-binding factor antibody
    see all

Anti-DDB1 antibody 画像

  • Anti-DDB1 antibody (ab97522) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 127 kDa
  • Anti-DDB1 antibody (ab97522) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

    Predicted band size : 127 kDa
  • ab97522 at 1/200 dilution staining DDB1 in Paraformaldehyde fixed HeLa cells. Lower image is merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using ab97522 at 1/500 dilution.

Anti-DDB1 antibody (ab97522) 使用論文

This product has been referenced in:
  • Parsons JL  et al. Phosphorylation of PNKP by ATM prevents its proteasomal degradation and enhances resistance to oxidative stress. Nucleic Acids Res : (2012). WB ; Human . Read more (PubMed: 23042680) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample Human Cell (Human primary fibroblasts)
Specification Human primary fibroblasts
Permeabilization Yes - 0.2% Triton-X100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount 15 µg
Specification Mouse embryonic fibroblasts
Gel Running Conditions Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Aug 26 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa cells)
Total protein in input 200 µg
Specification HeLa cells
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

投稿 Mar 18 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 20 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jan 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"