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Our Abpromise guarantee covers the use of ab1383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).Can be blocked with Human DDAH2 peptide (ab22866). Please note that DDAH2, according to NP_039268, has a consensus glycosylation site. Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].|
|IHC-FoFr||Use at an assay dependent dilution. PubMed: 21408057|
|ICC/IF||Use at an assay dependent dilution.|