Anti-DDAH1 抗体 (ab108088)
Key features and details
- Rabbit polyclonal to DDAH1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DDAH1 antibody
DDAH1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DDAH1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Cow, Dog, Pig, Macaque monkey, Chinese hamster -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human DDAH1.
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ポジティブ・コントロール
- WB: Wild-type A431 cell lysate. A431, HUVEC, HepG2 and HEK293 cell lysates; Human fetal liver, liver, kidney, brain and pancreas cell lystates. IHC-P: Human kidney tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108088の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa).
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa). |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. -
組織特異性
Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle. -
配列類似性
Belongs to the DDAH family. - Information by UniProt
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参照データベース
- Entrez Gene: 23576 Human
- Entrez Gene: 69219 Mouse
- Entrez Gene: 64157 Rat
- Omim: 604743 Human
- SwissProt: P56965 Cow
- SwissProt: O94760 Human
- SwissProt: Q9CWS0 Mouse
- SwissProt: O08557 Rat
see all -
別名
- DDAH antibody
- DDAH I antibody
- DDAH-1 antibody
see all
画像
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All lanes : Anti-DDAH1 antibody (ab108088) at 1 µg/ml
Lane 1 : Wild-type A431 cell lysate
Lane 2 : DDAH1 knockout A431 cell lysate
Lane 3 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 4 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab108088 observed at 35 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab108088 was shown to react with DDAH1 in wild-type A431 cells in western blot. Loss of signal was observed when DDAH1 knockout sample was used. Wild-type and DDAH1 knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108088 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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IHC image of DDAH1 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108088, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
All lanes : Anti-DDAH1 antibody (ab108088) at 1 µg/ml
Lane 1 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : Human kidney tissue lysate - total protein (ab30203)
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 6 : Human brain tissue lysate - total protein (ab29466)
Lane 7 : Human pancreas tissue lysate - total protein (ab29816)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 62 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab108088 は 1 報の論文で使用されています。
- Osbun JW et al. Comparative Proteomic Profiling Using Two-Dimensional Gel Electrophoresis and Identification via LC-MS/MS Reveals Novel Protein Biomarkers to Identify Aggressive Subtypes of WHO Grade I Meningioma. J Neurol Surg B Skull Base 78:371-379 (2017). PubMed: 28875114