製品の概要

  • 製品名Anti-DCXR antibody [4G4AF5]
    DCXR 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4G4AF5] to DCXR
  • アプリケーション適用あり: WB, ICC/IF, In-Cell ELISA, Flow Cyt, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human liver mitochondria

  • ポジティブ・コントロール
    • Isolated human liver mitochondria; Human HDFn cells; HL-60 cells.
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
ICC/IF Use a concentration of 4 µg/ml.
In-Cell ELISA Use a concentration of 4 µg/ml. (0.4 µg/well).
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules.
  • 組織特異性Highly expressed in kidney, liver and epididymis. In the epididymis, it is mainly expressed in the proximal and distal sections of the corpus region. Weakly or not expressed in brain, lung, heart, spleen and testis.
  • 関連疾患Note=The enzyme defect in pentosuria has been shown to involve L-xylulose reductase. Essential pentosuria is an inborn error of metabolism characterized by the excessive urinary excretion of the pentose L-xylulose.
  • 配列類似性Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • 細胞内局在Membrane. Probably recruited to membranes via an interaction with phosphatidylinositol.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Carbonyl reductase 2 antibody
    • Carbonyl reductase II antibody
    • DCR antibody
    • Dcxr antibody
    • DCXR_HUMAN antibody
    • Dicarbonyl L xylulose reductase antibody
    • Dicarbonyl/ L xylulose reductase antibody
    • Dicarbonyl/L-xylulose reductase antibody
    • HCR2 antibody
    • HCRII antibody
    • Human carbonyl reductase 2 antibody
    • kiDCR antibody
    • Kidney dicarbonyl reductase antibody
    • L xylulose reductase antibody
    • L-xylulose reductase antibody
    • P34H antibody
    • PNTSU antibody
    • SDR20C1 antibody
    • Short chain dehydrogenase/reductase family 20C member 1 antibody
    • Sperm surface protein P34H antibody
    • XR antibody
    see all

Anti-DCXR antibody [4G4AF5] 画像

  • Anti-DCXR antibody [4G4AF5] (ab110283) at 1 µg/ml + Human liver mitochondria at 20 µg

    Secondary
    Goat anti-mouse conjugated to AP

    Predicted band size : 26 kDa
  • Immunocytochemistry image of ab110283 stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110283 at 4 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in the cell membrane.
  • HL-60 cells were stained with 1 µg/mL ab110283 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Anti-DCXR antibody [4G4AF5] (ab110283) 使用論文

ab110283 has not yet been referenced specifically in any publications.

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