製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to DCTN1
  • 特異性
    This antibody is expected to recognise both human isoforms.
  • アプリケーション
    適用あり: IHC-P, ICC, ICC/IF, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Drosophila melanogaster
  • 免疫原

    Synthetic peptide: QEQLHQLHSRLIS, corresponding to amino acids 1266-1278 of Human DCTN1.

  • ポジティブ・コントロール
    • A549 and Human Testis lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent dilution. PubMed: 17932487
IHC-Fr Use at an assay dependent dilution. PubMed: 17932487
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 150 kDa.Can be blocked with Human DCTN1 peptide (ab23214). Approx 150kDa band observed in A549 and Human Testis lysates

ターゲット情報

  • 機能
    Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.
  • 組織特異性
    Brain.
  • 関連疾患
    Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B) [MIM:607641]; also known as progressive lower motor neuron disease (PLMND). HMN7B is a neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
    Defects in DCTN1 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
    Defects in DCTN1 are the cause of Perry syndrome (PERRYS) [MIM:168605]; also called parkinsonism with alveolar hypoventilation and mental depression. Perry syndrome is a neuropsychiatric disorder characterized by mental depression not responsive to antidepressant drugs or electroconvulsive therapy, sleep disturbances, exhaustion and marked weight loss. Parkinsonism develops later and respiratory failure occurred terminally.
  • 配列類似性
    Belongs to the dynactin 150 kDa subunit family.
    Contains 1 CAP-Gly domain.
  • 翻訳後修飾
    Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome.
  • 細胞内局在
    Cytoplasm. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • 150 kDa dynein associated polypeptide antibody
    • 150 kDa dynein-associated polypeptide antibody
    • DAP 150 antibody
    • DAP-150 antibody
    • DAP150 antibody
    • DCTN 1 antibody
    • DCTN1 antibody
    • DCTN1_HUMAN antibody
    • DP 150 antibody
    • DP-150 antibody
    • DP150 antibody
    • Dynactin 1 (p150 Glued (Drosophila) homolog) antibody
    • Dynactin 1 (p150 glued homolog Drosophila) antibody
    • Dynactin 1 antibody
    • Dynactin subunit 1 antibody
    • Dynactin1 antibody
    • HMN7B antibody
    • p135 antibody
    • p150 Glued (Drosophila) homolog antibody
    • p150 glued antibody
    • p150 glued homolog antibody
    • p150(GLUED) DROSOPHILA HOMOLOG OF antibody
    • p150-glued antibody
    • p150glued antibody
    see all

画像

  • Anti-DCTN1 antibody (ab11806) at 1 µg/ml + Human testis tissue lysate at 35 µg

    Predicted band size : 150 kDa


    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • ab11806 at 2.5ug/ml staining DCTN1 in human cerebellum tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

  • ab11806 staining DCTN1 in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.5% Triton X and blocked with 3% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/100) for 1 hour. An Alexa Fluor®488-conjugated Donkey anti-goat polyclonal (1/2000) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of murine colon tissue, staining DCTN1 with ab11806.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 5% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/200 in diluent) for 12 hours at 4°C. An AlexaFluor®488-conjugated donkey anti-goat polyclonal IgG (1/800) was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Chanduri M  et al. Inositol hexakisphosphate kinase 1 (IP6K1) activity is required for cytoplasmic dynein-driven transport. Biochem J 473:3031-47 (2016). Read more (PubMed: 27474409) »
  • Villarin JM  et al. Local synthesis of dynein cofactors matches retrograde transport to acutely changing demands. Nat Commun 7:13865 (2016). Read more (PubMed: 28000671) »

See all 7 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Colon)
Specification
Colon
Fixative
Paraformaldehyde
Permeabilization
Yes - .01% triton
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

投稿 Nov 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
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Verified customer

投稿 Aug 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF)
Specification
MEF
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X
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Verified customer

投稿 Mar 17 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (MEF)
Loading amount
50 µg
Specification
MEF
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

投稿 Feb 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (HELA)
Loading amount
50 µg
Specification
HELA
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Feb 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (HELA)
Specification
HELA
Fixative
Methanol
Permeabilization
Yes - 0.5% TRITON-x
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
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Abcam user community

Verified customer

投稿 Feb 11 2011

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with zebrafish has not yet been tested. Should you decide to go ahead and purchase this product, pleas...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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