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Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Dcp2.
(Peptide available as ab30458.)
Our Abpromise guarantee covers the use of ab28658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48.4 kDa).|
|IHC-P||1/35. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|EMSA||Use at an assay dependent concentration. PubMed: 20691152|
|IP||Use at an assay dependent concentration.|
ICC/IF image of ab28658 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab28658, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).