製品の概要

  • 製品名Anti-Dcp1a antibody
    Dcp1a 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Dcp1a
  • 特異性ab66009 detects endogenous levels of total Dcp1a protein.
  • アプリケーション適用あり: ICC/IF, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide derived from an internal sequence of human Dcp1a.

  • ポジティブ・コントロール
    • Jurkat cell extracts.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa).

ターゲット情報

  • 機能Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.
  • 組織特異性Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.
  • 配列類似性Belongs to the DCP1 family.
  • 細胞内局在Cytoplasm > P-body. Nucleus. Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DCP1 decapping enzyme homolog A antibody
    • Dcp1a antibody
    • DCP1A_HUMAN antibody
    • Decapping enzyme hDcp1a antibody
    • Decapping mRNA 1A antibody
    • HSA275986 antibody
    • mRNA decapping enzyme 1A antibody
    • mRNA-decapping enzyme 1A antibody
    • Nbla00360 antibody
    • Putative protein product of Nbla00360 antibody
    • Smad4 interacting transcriptional co activator antibody
    • Smad4-interacting transcriptional co-activator antibody
    • Smad4-interacting transcriptional co-activator antibody
    • SMAD4IP1 antibody
    • SMIF antibody
    • Transcription factor SMIF antibody
    see all

Anti-Dcp1a antibody 画像

  • All lanes : Anti-Dcp1a antibody (ab66009) at 1/500 dilution

    Lane 1 : Jurkat cell extracts
    Lane 2 : Jurkat cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 63 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab66009 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66009, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Dcp1a antibody (ab66009) 使用論文

ab66009 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (Mammary cell)
Loading amount 50 µg
Specification Mammary cell
Treatment TGF beta for 6 hrs
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Oct 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"