製品の概要

  • 製品名Anti-Dcp1a antibody
    Dcp1a 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Dcp1a
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: STQLSNLGST ETLEEMPSGS QDKSAPSGHK HLTVEELFGT SLPKEQPAVV GLDSEEMERL PGDASQKEPN SFLPFPFEQL GGAPQSETLG VPSAAHHSVQ , corresponding to amino acids 186-286 of Human Dcp1a

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab57654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 63 kDa.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.
  • 組織特異性Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.
  • 配列類似性Belongs to the DCP1 family.
  • 細胞内局在Cytoplasm > P-body. Nucleus. Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DCP1 decapping enzyme homolog A antibody
    • Dcp1a antibody
    • DCP1A_HUMAN antibody
    • Decapping enzyme hDcp1a antibody
    • Decapping mRNA 1A antibody
    • HSA275986 antibody
    • mRNA decapping enzyme 1A antibody
    • mRNA-decapping enzyme 1A antibody
    • Nbla00360 antibody
    • Putative protein product of Nbla00360 antibody
    • Smad4 interacting transcriptional co activator antibody
    • Smad4-interacting transcriptional co-activator antibody
    • Smad4-interacting transcriptional co-activator antibody
    • SMAD4IP1 antibody
    • SMIF antibody
    • Transcription factor SMIF antibody
    see all

Anti-Dcp1a antibody 画像



  • Predicted band size : 63 kDa
    DCP1A antibody (ab57654) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.
  • Overlay histogram showing HepG2 cells stained with ab57654 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57654, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Dcp1a antibody (ab57654) 使用論文

This product has been referenced in:
  • Nishi K  et al. Control of the localization and function of a miRNA silencing component TNRC6A by Argonaute protein. Nucleic Acids Res 43:9856-73 (2015). Read more (PubMed: 26446993) »
  • Blanco FF  et al. Transforming growth factor ß regulates P-body formation through induction of the mRNA decay factor tristetraprolin. Mol Cell Biol 34:180-95 (2014). Human . Read more (PubMed: 24190969) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T cell)
Loading amount 20 µg
Specification 293T cell
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"