製品の概要

  • 製品名
    Anti-DCK antibody [OTI16E12]
    DCK 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [OTI16E12] to DCK
  • アプリケーション
    適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein corresponding to Human DCK (NP_000779) produced in HEK293T cells.

  • ポジティブ・コントロール
    • HEK293T cells transfected with pCMV6-ENTRY DCK. HeLa cells. Jurkat cells.
  • 特記事項

    Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

    The clone number has been updated from 16E12 to OTI16E12, both clone numbers name the same clone.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    Purified from mouse ascites fluids by affinity chromatography.
  • ポリ/モノ
    モノクローナル
  • クローン名
    OTI16E12
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab118994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 31 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
  • 配列類似性
    Belongs to the DCK/DGK family.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dCK antibody
    • DCK protein antibody
    • DCK_HUMAN antibody
    • Deoxycytidine kinase antibody
    • Deoxynucleoside kinase antibody
    • EC 2.7.1 antibody
    • EC 2.7.1.74 antibody
    • MGC117410 antibody
    • MGC138632 antibody
    • OTTHUMP00000219118 antibody
    • OTTHUMP00000219119 antibody
    see all

画像

  • All lanes : Anti-DCK antibody [OTI16E12] (ab118994) at 1/2000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY DCK cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 31 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210767)
  • HEK293T cells transfected with either DCK overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab118994 at 1/100, and then analyzed by flow cytometry.
  • Flow cytometric analysis of HeLa cells, using ab118994 at 1/100 (Red) compared to a nonspecific negative control antibody (Blue).
  • Flow cytometric analysis of Jurkat cells, using ab118994 at 1/100 (Red), compared to a nonspecific negative control antibody (Blue).

参考文献

ab118994 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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