製品の概要
-
製品名Anti-DCK antibody
DCK 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to DCK
-
由来種Rabbit
-
アプリケーション適用あり: WB, ICC/IFmore details
-
種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
-
免疫原
Recombinant fragment corresponding to a region within amino acids 1 - 216 of Human DCK.
-
ポジティブ･コントロール
- 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lines.
法規制情報
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
バッファーPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0 -
Concentration information loading... -
精製度Immunogen affinity purified
-
ポリ/モノポリクローナル
-
アイソタイプIgG
-
研究分野
関連製品
-
Compatible Secondaries
-
Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab96599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 31 kDa. | |
| ICC/IF | 1/100 - 1/200. |
ターゲット情報
-
機能Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
-
配列類似性Belongs to the DCK/DGK family.
-
翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
-
細胞内局在Nucleus.
- Information by UniProt
-
参照データベース
- Entrez Gene: 1633 Human
- Entrez Gene: 13178 Mouse
- Entrez Gene: 79127 Rat
- Omim: 125450 Human
- SwissProt: Q3MHR2 Cow
- SwissProt: P27707 Human
- SwissProt: P43346 Mouse
- SwissProt: P48769 Rat
see all -
別名
- dCK antibody
- DCK protein antibody
- DCK_HUMAN antibody
see all
画像
-
Western blot - Anti-DCK antibody (ab96599)Anti-DCK antibody (ab96599) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 31 kDa
12% SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-DCK antibody (ab96599)ab96599, at 1/200 dilution, staining DCK in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.
参考文献
This product has been referenced in:
- Hammam K et al. Dual protein kinase and nucleoside kinase modulators for rationally designed polypharmacology. Nat Commun 8:1420 (2017). Read more (PubMed: 29127277) »
- Kerr M et al. Deoxycytidine kinase expression underpins response to gemcitabine in bladder cancer. Clin Cancer Res 20:5435-45 (2014). WB . Read more (PubMed: 25224279) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"