製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DCK
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment corresponding to a region within amino acids 1 - 216 of Human DCK.

  • ポジティブ・コントロール
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lines.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 31 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能
    Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
  • 配列類似性
    Belongs to the DCK/DGK family.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dCK antibody
    • DCK protein antibody
    • DCK_HUMAN antibody
    • Deoxycytidine kinase antibody
    • Deoxynucleoside kinase antibody
    • EC 2.7.1 antibody
    • EC 2.7.1.74 antibody
    • MGC117410 antibody
    • MGC138632 antibody
    • OTTHUMP00000219118 antibody
    • OTTHUMP00000219119 antibody
    see all

画像

  • Anti-DCK antibody (ab96599) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 31 kDa
  • ab96599, at 1/200 dilution, staining DCK in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.

参考文献

This product has been referenced in:
  • Kerr M  et al. Deoxycytidine kinase expression underpins response to gemcitabine in bladder cancer. Clin Cancer Res 20:5435-45 (2014). WB . Read more (PubMed: 25224279) »
  • Frese KK  et al. nab-Paclitaxel potentiates gemcitabine activity by reducing cytidine deaminase levels in a mouse model of pancreatic cancer. Cancer Discov 2:260-269 (2012). Read more (PubMed: 22585996) »

See all 2 Publications for this product

レビューと Q&A

rdetails and for your cooperation.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused.
I appreciate the time you have spent on these experiments in the laboratory and it is disappointi...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (murine pancreatic adenocarcinoma)
Specification
murine pancreatic adenocarcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Kris Frese

Verified customer

投稿 Jan 23 2012

Application
Western blot
Sample
Mouse Tissue lysate - whole (multiple tissues)
Loading amount
40 µg
Specification
multiple tissues
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Kris Frese

Verified customer

投稿 Jan 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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