製品の概要

  • 製品名Anti-DCK antibody [16E12]
    DCK 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [16E12] to DCK
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein corresponding to Human DCK (NP_000779) produced in HEK293T cells.

  • ポジティブ・コントロール
    • HEK293T cells transfected with pCMV6-ENTRY DCK. HeLa cells. Jurkat cells.
  • 特記事項Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)Purified from mouse ascites fluids by affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名16E12
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab118994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 31 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
  • 配列類似性Belongs to the DCK/DGK family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dCK antibody
    • DCK protein antibody
    • DCK_HUMAN antibody
    • Deoxycytidine kinase antibody
    • Deoxynucleoside kinase antibody
    • EC 2.7.1 antibody
    • EC 2.7.1.74 antibody
    • MGC117410 antibody
    • MGC138632 antibody
    • OTTHUMP00000219118 antibody
    • OTTHUMP00000219119 antibody
    see all

Anti-DCK antibody [16E12] 画像

  • All lanes : Anti-DCK antibody [16E12] (ab118994) at 1/2000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY DCK cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 31 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210767)
  • HEK293T cells transfected with either DCK overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab118994 at 1/100, and then analyzed by flow cytometry.
  • Flow cytometric analysis of HeLa cells, using ab118994 at 1/100 (Red) compared to a nonspecific negative control antibody (Blue).
  • Flow cytometric analysis of Jurkat cells, using ab118994 at 1/100 (Red), compared to a nonspecific negative control antibody (Blue).

Anti-DCK antibody [16E12] (ab118994) 使用論文

ab118994 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"