Anti-DCK 抗体 [OTI16E12] (ab118994)
Key features and details
- Mouse monoclonal [OTI16E12] to DCK
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-DCK antibody [OTI16E12]
DCK 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [OTI16E12] to DCK -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human DCK aa 1-300. (NP_000779) produced in HEK293T cells.
Database link: P27707 -
ポジティブ・コントロール
- WB: HEK293T cells transfected with pCMV6-ENTRY DCK. Flow Cyt (Intra): HEK-293T transfected with DCK, HeLa and Jurkat cells.
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特記事項
The clone number has been updated from 16E12 to OTI16E12, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Purified from cell culture supernatant by affinity chromatography -
ポリ/モノ
モノクローナル -
クローン名
OTI16E12 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118994の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB |
1/2000. Predicted molecular weight: 31 kDa.
|
特記事項 |
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Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/2000. Predicted molecular weight: 31 kDa. |
ターゲット情報
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機能
Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. -
配列類似性
Belongs to the DCK/DGK family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1633 Human
- Omim: 125450 Human
- SwissProt: P27707 Human
- Unigene: 709 Human
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別名
- dCK antibody
- DCK protein antibody
- DCK_HUMAN antibody
see all
画像
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All lanes : Anti-DCK antibody [OTI16E12] (ab118994) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY DCK cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 31 kDa -
Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either DCK overexpress plasmid (Red) or empty vector control plasmid (Blue) were stained for DCK using ab118994 at 1/100 dilution.
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Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DCK using ab118994 (Red) compared to a nonspecific negative control antibody (Blue).
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Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells using ab118994 at 1/100 (Red), compared to a nonspecific negative control antibody (Blue).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab118994 は論文での使用が確認できていません。