The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 50-82 kDa (predicted molecular weight: 82 kDa).
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/250.
Is unsuitable for Flow Cyt or IP.
Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 2 doublecortin domains. Contains 1 protein kinase domain.
Calcium/calmodulin-dependent protein kinase type I-like CPG16 antibody
Doublecortin domain-containing protein 3A antibody
Doublecortin-like and CAM kinase-like 1 antibody
Doublecortin-like kinase 1 antibody
Serine/threonine-protein kinase DCAMKL1 antibody
Serine/threonine-protein kinase DCLK1 antibody
Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)
Predicted band size : 82 kDa
Lane 1: Wild-type HAP1 whole cell lysate (20 µg) Lane 2: Knockout HAP1 whole cell lysate (20 µg) Lane 3: DCLK1 (KO) whole cell lysate (20 µg) Lane 4: Empty whole cell lysate (20 µg) Lane 5: Hu brain whole cell lysate (20 µg) Lanes 1 - 5: Merged signal (red and green). Green - ab109029 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109029 was shown to specifically react with DCLK1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when knockout samples were used. Wild-type and knockout samples were subjected to SDS-PAGE. Ab109029 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - DCAMKL1 antibody [EPR6085] (ab109029)
All lanes : Anti-DCAMKL1 antibody [EPR6085] (ab109029) at 1/1000 dilution
Lane 1 : Human fetal lung lysate Lane 2 : NIH3T3 lysate Lane 3 : SH-SY5Y lysate