Anti-DCAMKL1 抗体 (ab88484)

製品の概要

  • 製品名Anti-DCAMKL1 antibody
    DCAMKL1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to DCAMKL1
  • アプリケーション適用あり: WB, ELISA, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Recombinant Fragment
    交差が予測される動物種: Rat, Human, Chimpanzee, Rhesus monkey
  • 免疫原

    Recombinant fragment: QVLEHPWVND DGLPENEHQL SVAGKIKKHF NTGPKPNSTA AGVSVIALDH GFTIKRSGSL DYYQQPGMYW IRPPLLIRRG RFSDEDATRM, corresponding to amino acids 640-729 of Human DCAMKL1 (NP_004725) with a proprietary tag.

  • ポジティブ・コントロール
    • Partial tagged recombinant DCAMKL1 protein. NIH/3T3 cell lysate. NIH/3T3 cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab88484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
  • 組織特異性In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 2 doublecortin domains.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Calcium/calmodulin-dependent protein kinase type I-like CPG16 antibody
    • CL1 antibody
    • CLICK1 antibody
    • Cpg16 antibody
    • DCDC3A antibody
    • Dcl antibody
    • Dclk antibody
    • Dclk1 antibody
    • DCLK1_HUMAN antibody
    • Doublecortin domain-containing protein 3A antibody
    • Doublecortin-like and CAM kinase-like 1 antibody
    • Doublecortin-like kinase 1 antibody
    • KIAA0369 antibody
    • Serine/threonine-protein kinase DCAMKL1 antibody
    • Serine/threonine-protein kinase DCLK1 antibody
    see all

Anti-DCAMKL1 antibody 画像

  • Anti-DCAMKL1 antibody (ab88484) at 5 µg/ml + Partial tagged recombinant DCAMLK1 protein (immunogen) at 0.1 µg

    Predicted band size : 82 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Anti-DCAMKL1 antibody (ab88484) at 5 µg/ml + NIH/3T3 cell lysate at 50 µg

    Predicted band size : 82 kDa
    Observed band size : 73 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • ab88484, at 10 µg/ml, stainining DCAMKL1 in NIH/3T3 cells by Immunofluorescence.
  • IHC image of ab88484 staining in Human Hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88484, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-DCAMKL1 antibody (ab88484) 使用論文

ab88484 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Colon)
Specification Colon
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Sep 20 2010

There are two monoclonals which are specific for the AS isoform: 1. ab88484- The immunogen has 52% homology with AL isoform. 2. ab56482- has the same immunogen but it has only been tested against the immunogen in WB.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"