製品の概要

  • 製品名Anti-DCAMKL1 antibody
    DCAMKL1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DCAMKL1
  • 特異性This antibody recognizes 4 different human isoforms (AS: 81.1 KDa, AL: 82.2 KDa, BS: 46.5 KDa and BL: 47.6 KDa)
  • アプリケーション適用あり: ICC/IF, IHC-FoFr, IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Mouse DCAMLK1.

    (Peptide available as ab31703.)

  • ポジティブ・コントロール
    • WB: Mouse Brain, Rat Brain, Human Brain IHC: Rat brain (e.g. cortex, striatum)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab31704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IHC-FoFr 1/1000.
IHC-P Use at an assay dependent concentration. PubMed: 20522640
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 47, 82 kDa (predicted molecular weight: 47, 82 kDa).Can be blocked with Mouse DCAMKL1 peptide (ab31703).
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
  • 組織特異性In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 2 doublecortin domains.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Calcium/calmodulin-dependent protein kinase type I-like CPG16 antibody
    • CL1 antibody
    • CLICK1 antibody
    • Cpg16 antibody
    • DCDC3A antibody
    • Dcl antibody
    • Dclk antibody
    • Dclk1 antibody
    • DCLK1_HUMAN antibody
    • Doublecortin domain-containing protein 3A antibody
    • Doublecortin-like and CAM kinase-like 1 antibody
    • Doublecortin-like kinase 1 antibody
    • KIAA0369 antibody
    • Serine/threonine-protein kinase DCAMKL1 antibody
    • Serine/threonine-protein kinase DCLK1 antibody
    see all

Anti-DCAMKL1 antibody 画像

  • Anti-DCAMKL1 antibody (ab31704) at 1 µg/ml + Mouse Brain Whole Tissue Lysate at 20 µg

    Secondary
    IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 47, 82 kDa
    Observed band size : 47,82 kDa
    The 82 kDa and 47 kDa bands correspond to the AL and BL isoforms respectively. This antibody should not detect the AS and BS isoforms of DCAMLK1.
  • ICC/IF image of ab31704 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31704, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

  • Anti-DCAMKL1 antibody (ab31704) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 47, 82 kDa
    Observed band size : 47,82 kDa
  • ab31704 at a 1/1000 dilution staining DCAMKL1 in rat brain tissue sections (hippocampus) by Immunohistochemistry/ PFA perfused, frozen sections, incubated for 18 hours at 20°C in PBS + 0.3% Triton X-100. Secondary used at 1/1000 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 488.
    The immunostaining was performed using the `free floating` technique, using direct fluorescence. The antibody beautifully stains tracts of fibers in many brain areas such as the cortex, striatum. The image shown is staining observed at the level of the striatum.

    See Abreview

  • ICC/IF image of ab31704 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31704, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • Anti-DCAMKL1 antibody (ab31704) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 47, 82 kDa
    Observed band size : 47,82 kDa
    Additional bands at : 30 kDa,52 kDa (possible post-translational modification),54 kDa (possible post-translational modification). We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes
    The 82 kDa and 47 kDa bands correspond to the AL and BL isoforms respectively. DCAMLK1 has a number of potential phosphorylation sites which may explain the higher migrating bands at 52 and 54 kDa.
  • DCAMKL1 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to DCAMKL1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31704.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 82kDa: DCAMKL1; non specific - 52 and 27kDa: We are unsure as to the identity of this extra band.

Anti-DCAMKL1 antibody (ab31704) 使用論文

This product has been referenced in:
  • Qu D  et al. Doublecortin-like kinase 1 is elevated serologically in pancreatic ductal adenocarcinoma and widely expressed on circulating tumor cells. PLoS One 10:e0118933 (2015). WB ; Human . Read more (PubMed: 25723399) »
  • O'Connell MR  et al. Epigenetic changes and alternate promoter usage by human colon cancers for expressing DCLK1-isoforms: Clinical Implications. Sci Rep 5:14983 (2015). Human . Read more (PubMed: 26447334) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Sample Mouse Tissue lysate - other (Brain, Cortex)
Specification Brain, Cortex
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Aug 02 2013

Thank you for your phone call.

A suitable isotype control for ab31704 and ab37994 would be: ab27478

http://www.abcam.com/Rabbit-polyclonal-IgG-ab27478.html


Please find herethe discount code and more detailed informat...

Read More

Thank you for your enquiry. The 4 isoforms AL, BL, AS and BS are related to the human DCLK1 protein: Isoform 2 [http://www.uniprot.org/uniprot/O15075#O15075-1] (identifier: O15075-1) Also known as: AL; Isoform 1 [http://www.uniprot.org/uni...

Read More

There are two monoclonals which are specific for the AS isoform: 1. ab88484- The immunogen has 52% homology with AL isoform. 2. ab56482- has the same immunogen but it has only been tested against the immunogen in WB.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

投稿 Dec 03 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"