製品の概要

  • 製品名Anti-DBX1 antibody
    DBX1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DBX1
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Cow, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse DBX1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following mouse tissue lysates: E14 Embryo Brain; E14 Embryo Spinal Cord; E16 Embryo Brain; E16 Embryo Spinal Cord; E18 Embryo Brain; E18 Embryo Spinal Cord. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: NIH-3T3.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).

ターゲット情報

  • 関連性Dbx1 homeodomain transcription factor is expressed in progenitors at the boundary between the dorsal and ventral plates of the caudal neural tube, from which postmitotic cells migrate tangentially to their final destination. Dbx1 is implicated in patterning the central nervous system during embryogenesis. Cell fate allocation and cell diversity are determined at very early stages in progenitor cells at precise coordinates along the dorsoventral and anteroposterior axis. In the spinal cord, the spatially restricted expression of Dbx1 in progenitors is critical in establishing interneuron cell fates and helps coordinate diverse phenotypic features. In the telencephalon, Dbx1 is expressed in restricted progenitor domains at the borders of the developing pallium.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • dbx antibody
    • Developing brain homeobox 1 antibody
    • Developing brain homeobox protein 1 antibody
    • Homeobox protein DBX1 antibody

Anti-DBX1 antibody 画像

  • All lanes : Anti-DBX1 antibody (ab61488) at 1 µg/ml

    Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
    Lane 2 : E14 Ms Embryo Spinal Cord Tissue Lysate
    Lane 3 : E16 Ms Embryo Brain Tissue Lysate
    Lane 4 : E16 Ms Embryo Spinal Cord Tissue Lysate
    Lane 5 : E18 Ms Embryo Brain Tissue Lysate
    Lane 6 : E18 Ms Embryo Spinal Cord Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • ICC/IF image of ab61488 stained NIH-3T3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab61488 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DBX1 antibody (ab61488) 使用論文

ab61488 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 May 22 2014

Thank you for your reply.

I am sorry that this antibody did not perform as stated on the datasheet. As requested, I have asked our Finance department to issue a credit note for you

Credit note ID: ######

The credit note...

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Thank you for taking the time to complete our questionnaire. The details you have kindly provided will provide us with vital information for our monitoring of product quality.

I appreciate the time you have spent in the laboratory and unders...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the r...

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Thank you for your reply. Frozen sections are falling in our category of immunohistochemistry (IHC-Fr). Please keep this in mind when submitting your Abreview.
Mouse samples have been tested, but human, rat and cow samples are only predicted to re...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Zebra finch Tissue sections (embryonal spinal cord)
Specification embryonal spinal cord
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TX100 in PBS
Blocking step Roti-Immunoblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Dr. Robert Storm

Verified customer

投稿 Feb 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"