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RabMAb

Anti-DBF4 抗体 [EPR5635] (ab124707)

製品の概要

  • 製品名
    Anti-DBF4 antibody [EPR5635]
    DBF4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5635] to DBF4
  • アプリケーション
    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human DBF4 protein (Q9UBU7).

  • ポジティブ・コントロール
    • PC-3, Jurkat, SW480, and HepG2 cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 77 kDa.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • ターゲット情報

    • 機能
      Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
    • 組織特異性
      Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
    • 配列類似性
      Contains 2 BRCT domains.
      Contains 1 DBF4-type zinc finger.
    • 翻訳後修飾
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Activator of S phase kinase antibody
      • ASK antibody
      • CHIF antibody
      • Chiffon homolog A antibody
      • DBF4 antibody
      • DBF4 homolog (S. cerevisiae) antibody
      • DBF4 homolog antibody
      • DBF4 type zinc finger containing protein 1 antibody
      • DBF4 zinc finger A antibody
      • DBF4 zinc finger antibody
      • DBF4, S. cerevisiae, homolog of antibody
      • DBF4-type zinc finger-containing protein 1 antibody
      • DBF4A antibody
      • DBF4A_HUMAN antibody
      • Dumbbell former 4, S. cerevisiae, homolog of antibody
      • OTTHUMP00000161661 antibody
      • Protein DBF4 homolog A antibody
      • ZDBF1 antibody
      • Zinc finger, DBF-type containing 1 antibody
      see all

    Anti-DBF4 antibody [EPR5635] 画像

    • All lanes : Anti-DBF4 antibody [EPR5635] (ab124707) at 1/10000 dilution

      Lane 1 : PC-3 cell lysates
      Lane 2 : Jurkat cell lysates
      Lane 3 : SW480 cell lysates
      Lane 4 : HepG2 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 77 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    プロトコール

    Anti-DBF4 antibody [EPR5635] (ab124707) 使用論文

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (hEK293)
    Total protein in input
    300 µg
    Immuno-precipitation step
    Other - protein G dynabeads
    Specification
    hEK293
    Username

    Dr. Edyta Marcon

    Verified customer

    投稿 Jun 27 2015

    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (4-12 Bis tris)
    Sample
    Human Cell lysate - whole cell (293T)
    Specification
    293T
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    投稿 Aug 14 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T)
    Loading amount
    50 µg
    Specification
    HEK293T
    Gel Running Conditions
    Reduced Denaturing (8% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 27 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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