リコンビナント
RabMAb

Anti-DBF4 抗体 [EPR5635] (ab124707)

製品の概要

  • 製品名
    Anti-DBF4 antibody [EPR5635]
    DBF4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5635] to DBF4
  • アプリケーション
    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human DBF4 protein (Q9UBU7).

  • ポジティブ・コントロール
    • PC-3, Jurkat, SW480, and HepG2 cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 77 kDa.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • ターゲット情報

    • 機能
      Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
    • 組織特異性
      Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
    • 配列類似性
      Contains 2 BRCT domains.
      Contains 1 DBF4-type zinc finger.
    • 翻訳後修飾
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Activator of S phase kinase antibody
      • ASK antibody
      • CHIF antibody
      • Chiffon homolog A antibody
      • DBF4 antibody
      • DBF4 homolog (S. cerevisiae) antibody
      • DBF4 homolog antibody
      • DBF4 type zinc finger containing protein 1 antibody
      • DBF4 zinc finger A antibody
      • DBF4 zinc finger antibody
      • DBF4, S. cerevisiae, homolog of antibody
      • DBF4-type zinc finger-containing protein 1 antibody
      • DBF4A antibody
      • DBF4A_HUMAN antibody
      • Dumbbell former 4, S. cerevisiae, homolog of antibody
      • OTTHUMP00000161661 antibody
      • Protein DBF4 homolog A antibody
      • ZDBF1 antibody
      • Zinc finger, DBF-type containing 1 antibody
      see all

    Anti-DBF4 antibody [EPR5635] 画像

    • All lanes : Anti-DBF4 antibody [EPR5635] (ab124707) at 1/10000 dilution

      Lane 1 : PC-3 cell lysates
      Lane 2 : Jurkat cell lysates
      Lane 3 : SW480 cell lysates
      Lane 4 : HepG2 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 77 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    プロトコール

    Anti-DBF4 antibody [EPR5635] (ab124707) 使用論文

    ab124707 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (hEK293)
    Total protein in input
    300 µg
    Immuno-precipitation step
    Other - protein G dynabeads
    Specification
    hEK293
    Username

    Dr. Edyta Marcon

    Verified customer

    投稿 Jun 27 2015

    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (4-12 Bis tris)
    Sample
    Human Cell lysate - whole cell (293T)
    Specification
    293T
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    投稿 Aug 14 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T)
    Loading amount
    50 µg
    Specification
    HEK293T
    Gel Running Conditions
    Reduced Denaturing (8% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 27 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録