製品の概要

  • 製品名Anti-DBF4 antibody
    DBF4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DBF4
  • 特異性ab70134 detects endogenous levels of total DBF4 protein.
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide derived from the N terminus of human DBF4.

  • ポジティブ・コントロール
    • NIH3T3 cell extracts treated with H2O2 (100µM, 30mins), COS7 cell extracts treated with PMA (125mg/ml, 30mins), and NIH3T3 cell extracts treated with both H2O2 (100µM, 30mins) and PMA (125mg/ml, 30mins).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab70124 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab70134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
ELISA 1/10000.

ターゲット情報

  • 機能Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
  • 組織特異性Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
  • 配列類似性Contains 2 BRCT domains.
    Contains 1 DBF4-type zinc finger.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activator of S phase kinase antibody
    • ASK antibody
    • CHIF antibody
    • Chiffon homolog A antibody
    • DBF4 antibody
    • DBF4 homolog (S. cerevisiae) antibody
    • DBF4 homolog antibody
    • DBF4 type zinc finger containing protein 1 antibody
    • DBF4 zinc finger A antibody
    • DBF4 zinc finger antibody
    • DBF4, S. cerevisiae, homolog of antibody
    • DBF4-type zinc finger-containing protein 1 antibody
    • DBF4A antibody
    • DBF4A_HUMAN antibody
    • Dumbbell former 4, S. cerevisiae, homolog of antibody
    • OTTHUMP00000161661 antibody
    • Protein DBF4 homolog A antibody
    • ZDBF1 antibody
    • Zinc finger, DBF-type containing 1 antibody
    see all

Anti-DBF4 antibody 画像

  • All lanes : Anti-DBF4 antibody (ab70134) at 1/500 dilution

    Lane 1 : NIH3T3 cell extracts treated with H2O2 (100µM, 30mins) at 5 µg
    Lane 2 : COS7 cell extracts treated with PMA (125mg/ml, 30mins)
    Lane 3 : NIH3T3 cell extracts treated with H2O2 (100µM, 30mins) and PMA (125mg/ml, 30mins) with immunising peptide at 5 µg


    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab70134 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DBF4 antibody (ab70134) 使用論文

ab70134 has not yet been referenced specifically in any publications.

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Thank you for contacting us in regards to Anti-DBF4 antibody (ab70134). I have been assured by the source of this antibody that the Western blot performed and displayed on the datasheet was performed with endogenous expression of DBF4 from NIH3T3 a...

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