製品の概要

  • 製品名Anti-Daxx (phospho S668) antibody
    Daxx 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Daxx (phospho S668)
  • 特異性This antibody detects endogenous levels of Daxx only when phosphorylated at serine 668.
  • アプリケーション適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human Daxx around the phosphorylation site of serine 668 (L-P-SP-P-P).

  • ポジティブ・コントロール
    • Extracts form 293 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
ELISA 1/10000.

ターゲット情報

  • 機能Transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Down-regulates basal and activated transcription. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Inhibits transcriptional activation of PAX3 and ETS1 through direct protein-protein interactions. Modulates PAX5 activity; the function seems to involve CREBBP. Acts as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, resulting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization. Acts as histone chaperone that facilitates deposition of histone H3.3. Acts as targeting component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Does not affect the ATPase activity of ATRX but alleviates its transcription repression activity. Upon neuronal activation associates with regulatory elements of selected immediate early genes where it promotes deposition of histone H3.3 which may be linked to transcriptional induction of these genes. Required for the recruitment of histone H3.3:H4 dimers to PML-nuclear bodies (PML-NBs); the process is independent of ATRX and facilitated by ASF1A; PML-NBs are suggested to function as regulatory sites for the incorporation of newly synthesized histone H3.3 into chromatin. In case of overexpression of centromeric histone variant CENPA (as found in various tumors) is involved in its mislocalization to chromosomes; the ectopic localization involves a heterotypic tetramer containing CENPA, and histones H3.3 and H4 and decreases binding of CTCF to chromatin. Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Shows restriction activity towards human cytomegalovirus (HCMV).
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the DAXX family.
  • ドメインThe Sumo interaction motif mediates Sumo binding, and is required both for sumoylation and binding to sumoylated targets.
  • 翻訳後修飾Sumoylated with SUMO1 on multiple lysine residues.
    Phosphorylated by HIPK1 upon glucose deprivation.
    Polyubiquitinated; which is promoted by CUL3 and SPOP and results in proteasomal degradation. Ubiquitinated by MDM2; inducing its degradation. Deubiquitinated by USP7; leading to stabilize it.
  • 細胞内局在Nucleus. Diffuse nuclear distribution pattern and no comparable dot-like accumulation of isoform 1 and Cytoplasm. Nucleus, nucleoplasm. Nucleus, PML body. Nucleus, nucleolus. Chromosome, centromere. Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli (Probable). Colocalizes with histone H3.3, ATRX, HIRA and ASF1A at PML-nuclear bodies (PubMed:12953102, PubMed:14990586, PubMed:23222847, PubMed:24200965). Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres (PubMed:9645950). Detected in cytoplasmic punctate structures (PubMed:11842083). Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress (PubMed:12968034). Colocalizes with RASSF1 in the nucleus (PubMed:18566590). Colocalizes with USP7 in nucleoplasma with accumulation in speckled structures (PubMed:16845383).
  • Information by UniProt
  • 参照データベース
  • 別名
    • antibody
    • BING 2 antibody
    • BING2 antibody
    • CENP-C binding protein antibody
    • DAP 6 antibody
    • DAP6 antibody
    • Daxx antibody
    • DAXX_HUMAN antibody
    • Death associated protein 6 antibody
    • Death domain associated protein 6 antibody
    • Death domain associated protein antibody
    • Death domain-associated protein 6 antibody
    • EAP 1 antibody
    • EAP1 antibody
    • ETS1 associated protein 1 antibody
    • ETS1-associated protein 1 antibody
    • Fas binding protein antibody
    • Fas death domain associated protein antibody
    • Fas death domain-associated protein antibody
    • hDaxx antibody
    • MGC126245 antibody
    • MGC126246 antibody
    see all

Anti-Daxx (phospho S668) antibody 画像

  • All lanes : Anti-Daxx (phospho S668) antibody (ab55323) at 1/500 dilution

    Lane 1 : Extracts from 293 cells treated with PBS for 60 minutes.
    Lane 2 : Extracts from 293 cells treated with PBS for 60 minutes, and with the immunising phosphopeptide.


    Predicted band size : 82 kDa
    Observed band size : 82 kDa
  • Ab55323 staining human normal placenta tissue. Staining is localised to nuclear and cytoplasmic compartments.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Daxx (phospho S668) antibody (ab55323) 使用論文

ab55323 has not yet been referenced specifically in any publications.

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