Daudi whole cell lysate (ab3951)

製品の概要

  • 製品名
    Daudi whole cell lysate
  • 特記事項
    Cell line: Daudi (Human Burkitt's lymphoma).
    Growth media: RPMI 1640 medium with 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, and 10% fetal bovine serum.


    Daudi cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • アプリケーション
    適用あり: WBmore details

法規制情報

製品の特性

  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Constituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • 精製度
    Whole Cell Lysate
  • ライゼート 備考
    Daudi cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • 研究分野
  • 背景
    Daudi cells are lymphoblast-like in morphology and contain the Epstein-Barr virus. They are positive for the Fc receptor and certain tumor markers and are used for studies of differentiation and tumorigenicity and for antitumour testing. The lysate is a positive control for: IKKalpha (numerous ab in our catalogue for IKK alpha), IKKbeta (=IKK2) (numerous ab in our catalogue for IKK beta), DR4 (numerous ab in our catalogue for DR4), NFkB p65 (numerous ab in our catalogue for NFkB p65).

アプリケーション

Our Abpromise guarantee covers the use of ab3951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Daudi cell lysate is ready to load on SDS-PAGE for Western blotting, 10 - 20 µg per lane is recommended for mini gel.

Daudi whole cell lysate (ab3951) 使用論文

ab3951 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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