製品の概要

  • 製品名Anti-DATF1 antibody [3B1]
    DATF1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3B1] to DATF1
  • アプリケーション適用あり: WB, ELISA, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to Human DATF1 aa 321-420.
    Sequence:

    ILQVQDETHSETADQQEAKWRPGDADGTDCTSIGTIEQKSSEDQGIKGRI EKAANPSGKKKLKIFQPVIEAPGASKCIGPGCCHVAQPDSVYCSNDCILK


    Database link: Q9BTC0

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 244 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Putative transcription factor, weakly pro-apoptotic when overexpressed (By similarity). Tumor suppressor.
  • 組織特異性Ubiquitous.
  • 配列類似性Contains 1 PHD-type zinc finger.
    Contains 1 TFIIS central domain.
  • 細胞内局在Cytoplasm. Nucleus. Translocates to the nucleus after pro-apoptotic stimuli.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BYE 1 antibody
    • BYE1 antibody
    • C20orf158 antibody
    • DATF 1 antibody
    • DATF-1 antibody
    • Death associated transcription factor 1 antibody
    • Death inducer obliterator 1 antibody
    • Death-associated transcription factor 1 antibody
    • Death-inducer obliterator 1 antibody
    • DIDO 1 antibody
    • DIDO 2 antibody
    • DIDO 3 antibody
    • DIDO1 antibody
    • DIDO1_HUMAN antibody
    • DIDO2 antibody
    • DIDO3 antibody
    • DIO 1 antibody
    • DIO-1 antibody
    • DIO1 antibody
    • dJ885L7.8 antibody
    • DKFZp434P1115 antibody
    • FLJ11265 antibody
    • hDido 1 antibody
    • hDido1 antibody
    • KIAA0333 antibody
    • MGC16140 antibody
    see all

Anti-DATF1 antibody [3B1] 画像

  • Anti-DATF1 antibody [3B1] (ab92868) at 5 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG HRP conjugated at 1/2500 dilution

    Predicted band size : 244 kDa
  • Overlay histogram showing HepG2 cells stained with ab92868 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92868, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunofluorescence analysis of ab92868 on HeLa cell at concentration 10 ug/ml

  • Anti-DATF1 antibody [3B1] (ab92868) at 5 µg/ml + immunogen (80aa recombinant fragment with a tag of 26kDa) at 0.2 µg

    Secondary
    Goat anti-Mouse IgG HRP conjugated at 1/5000 dilution

    Predicted band size : 244 kDa

Anti-DATF1 antibody [3B1] (ab92868) 使用論文

This product has been referenced in:
  • Sánchez de Diego A  et al. Dido3-dependent HDAC6 targeting controls cilium size. Nat Commun 5:3500 (2014). WB ; Mouse . Read more (PubMed: 24667272) »
  • Alonso-Orgaz S  et al. Proteomic characterization of human coronary thrombus in patients with ST-segment elevation acute myocardial infarction. J Proteomics 109C:368-381 (2014). WB ; Human . Read more (PubMed: 25065646) »

See all 2 Publications for this product

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