製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DARPP32
  • 特異性
    Detects endogenous levels of total DARPP-32 protein.
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic non-phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P)

  • ポジティブ・コントロール
    • Extracts from 293 cells treated with EGF, human brain tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.

ターゲット情報

  • 機能
    Inhibitor of protein-phosphatase 1.
  • 配列類似性
    Belongs to the protein phosphatase inhibitor 1 family.
  • 翻訳後修飾
    Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
    Phosphorylation of Thr-34 is required for activity.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DARPP 32 antibody
    • DARPP-32 antibody
    • Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
    • Dopamine and cAMP regulated neuronal phosphoprotein antibody
    • Dopamine and cAMP regulated phosphoprotein antibody
    • Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
    • Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
    • Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
    • FLJ20940 antibody
    • IPPD antibody
    • Neuronal phosphoprotein DARPP 32 antibody
    • PPP1R1B antibody
    • PPR1B_HUMAN antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
    • Protein phosphatase 1 regulatory subunit 1B antibody
    see all

画像

  • All lanes : Anti-DARPP32 antibody (ab51139) at 1/500 dilution

    Lane 1 : extracts from 293 cells treated with EGF
    Lane 2 : extracts from 293 cells treated with EGF with immunizing peptide

    Lysates/proteins at 0.2 µg/ml per lane.


    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • Ab51139 at 1/50 dilution staining human brain tissue, with and without blocking peptide; paraffin embedded.

参考文献

ab51139 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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