製品の概要

  • 製品名Anti-DAPP1 (phospho Y139) antibody
    DAPP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DAPP1 (phospho Y139)
  • 特異性ab63396 detects endogenous levels of DAPP1 only when phosphorylated at tyrosine 139.
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human DAPP1 around the phosphorylation site of tyrosine 139 (S-I-YP-E-S).

  • ポジティブ・コントロール
    • WB: 293 cell extracts treated with Insulin (0.01U/ml, 2mins). ICC/IF: A549 cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab63396 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab63396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Predicted molecular weight: 32 kDa.
ELISA 1/5000.

ターゲット情報

  • 機能May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.
  • 組織特異性Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.
  • 配列類似性Contains 1 PH domain.
    Contains 1 SH2 domain.
  • 翻訳後修飾Phosphorylated on tyrosine residues.
  • 細胞内局在Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B cell adapter molecule of 32 kDa antibody
    • B lymphocyte adapter protein Bam32 antibody
    • B-cell adapter molecule of 32 kDa antibody
    • BAM32 antibody
    • DAPP1 antibody
    • DAPP1_HUMAN antibody
    • DKFZp667E0716 antibody
    • Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide antibody
    • Dual adaptor of phosphotyrosine and 3 phosphoinositides antibody
    • hDAPP1 antibody
    see all

Anti-DAPP1 (phospho Y139) antibody 画像

  • ab63396, at 1/500 dilution, staining DAPP1 in A549 cells by Immunofluorescence, in the absence (left image) or presence (right image) of the immunising peptide.
  • All lanes : Anti-DAPP1 (phospho Y139) antibody (ab63396) at 1/500 dilution

    Lane 1 : 293 cell extracts treated with Insulin (0.01U/ml, 2mins)
    Lane 2 : 293 cell extracts treated with Insulin (0.01U/ml, 2mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 32 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

Anti-DAPP1 (phospho Y139) antibody (ab63396) 使用論文

ab63396 has not yet been referenced specifically in any publications.

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