製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to DAPP1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Dog
  • 免疫原

    Peptide with sequence C-

    KLSQIRKQLNQGE

    , corresponding to C terminal amino acids 257-269 from the internal region (near C Terminus) of Human DAPP1 (NP_055210.2)

  • ポジティブ・コントロール
    • Transfected HEK293 cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab123498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).

ターゲット情報

  • 機能
    May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.
  • 組織特異性
    Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.
  • 配列類似性
    Contains 1 PH domain.
    Contains 1 SH2 domain.
  • 翻訳後修飾
    Phosphorylated on tyrosine residues.
  • 細胞内局在
    Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B cell adapter molecule of 32 kDa antibody
    • B lymphocyte adapter protein Bam32 antibody
    • B-cell adapter molecule of 32 kDa antibody
    • BAM32 antibody
    • DAPP1 antibody
    • DAPP1_HUMAN antibody
    • DKFZp667E0716 antibody
    • Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide antibody
    • Dual adaptor of phosphotyrosine and 3 phosphoinositides antibody
    • hDAPP1 antibody
    see all

画像

  • Lane 1 : Anti-DAPP1 antibody (ab123498) at 0.1 µg/ml
    Lane 2 : Anti-DAPP1 antibody (ab123498) at 1 mg/ml
    Lane 3 : Anti-DYKDDDDK Tag antibody at 1/3000 dilution

    Lane 1 : HEK293 lysate in RIPA buffer over expressing Human DAPP1 with DYKDDDDK tag
    Lane 2 : Mock-transfected HEK293
    Lane 3 : HEK293 lysate in RIPA buffer over expressing Human DAPP1 with DYKDDDDK tag


    Predicted band size : 32 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab123498 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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