Anti-P97/DAP5 抗体 (ab85963)
Key features and details
- Rabbit polyclonal to P97/DAP5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-P97/DAP5 antibody
P97/DAP5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to P97/DAP5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
免疫原
Synthetic peptide corresponding to Human P97/DAP5 aa 800-850.
Database link: NP_001409.1 -
ポジティブ・コントロール
- HeLa cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85963の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 102 kDa.
|
IP | (1) |
Use at 5-10 µg/mg of lysate.
|
特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 102 kDa. |
IP
Use at 5-10 µg/mg of lysate. |
ターゲット情報
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機能
Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases. -
組織特異性
Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney. -
配列類似性
Belongs to the eIF4G family.
Contains 1 MI domain.
Contains 1 MIF4G domain.
Contains 1 W2 domain. -
翻訳後修飾
Phosphorylation; hyperphosphorylated during mitosis. - Information by UniProt
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参照データベース
- Entrez Gene: 286870 Cow
- Entrez Gene: 1982 Human
- Entrez Gene: 13690 Mouse
- Entrez Gene: 361628 Rat
- Omim: 602325 Human
- SwissProt: P78344 Human
- SwissProt: Q62448 Mouse
- SwissProt: P79398 Rabbit
see all -
別名
- DAP-5 antibody
- DAP5 antibody
- Death associated protein 5 antibody
see all
画像
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All lanes : Anti-P97/DAP5 antibody (ab85963) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 102 kDa
Exposure time: 3 minutes -
1mg HeLa whole cell lysate was immunoprecipitated using 10µg ab85963.
20% of IP was loaded per lane and probed with ab85963 at 0.4µg/ml (lane 1) and a control IgG (lane 2).
Detection: chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab85963 は 1 報の論文で使用されています。
- Xie JD et al. Presynaptic N-Methyl-d-aspartate (NMDA) Receptor Activity Is Increased Through Protein Kinase C in Paclitaxel-induced Neuropathic Pain. J Biol Chem 291:19364-73 (2016). PubMed: 27458019