製品の概要

  • 製品名Anti-DAP5 antibody
    DAP5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DAP5
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 144-193 (PNFDGPAAEG QPGQKQSTTF RRLLISKLQD EFENRTRNVD VYDKRENPLL) of human DAP5 (NP_001409).

  • ポジティブ・コントロール
    • 721_B cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 102 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

ターゲット情報

  • 機能Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
  • 組織特異性Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.
  • 配列類似性Belongs to the eIF4G family.
    Contains 1 MI domain.
    Contains 1 MIF4G domain.
    Contains 1 W2 domain.
  • 翻訳後修飾Phosphorylation; hyperphosphorylated during mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DAP-5 antibody
    • Death associated protein 5 antibody
    • Death-associated protein 5 antibody
    • eIF-4-gamma 2 antibody
    • eIF-4G 2 antibody
    • eIF4G 2 antibody
    • EIF4G2 antibody
    • Eukaryotic translation initiation factor 4 gamma 2 antibody
    • IF4G2_HUMAN antibody
    • Nat1 antibody
    • Novel APOBEC-1 target 1 antibody
    • p97 antibody
    • Translation repressor NAT1 antibody
    • Translation repressor NAT1 antibody
    see all

Anti-DAP5 antibody 画像

  • Anti-DAP5 antibody (ab83358) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 102 kDa
    Observed band size : 135 kDa (why is the actual band size different from the predicted?)

Anti-DAP5 antibody (ab83358) 使用論文

ab83358 has not yet been referenced specifically in any publications.

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