製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to DAP12
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Sheep, Dog
  • 免疫原

    Synthetic peptide:

    KQRITETESPYQE

    , corresponding to amino acids 81-93 of Human DAP12

  • ポジティブ・コントロール
    • Human Spleen and Human Uterus tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab110117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation.
  • 組織特異性
    Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum.
  • 関連疾患
    Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
  • 配列類似性
    Belongs to the TYROBP family.
  • 翻訳後修飾
    Tyrosine phosphorylated.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DAP 12 antibody
    • DAP12 antibody
    • DNAX activation protein 12 antibody
    • DNAX-activation protein 12 antibody
    • KAR-associated protein antibody
    • KARAP antibody
    • Killer activating receptor associated protein antibody
    • Killer-activating receptor-associated protein antibody
    • PLOSL antibody
    • TYOBP_HUMAN antibody
    • TYRO protein tyrosine kinase binding protein antibody
    • TYRO protein tyrosine kinase-binding protein antibody
    • TYROBP antibody
    see all

画像

  • Human Spleen (formalin-fixed, paraffin-embedded) stained with 3 µg/ml ab110117 followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Human Uterus (formalin-fixed, paraffin-embedded) stained with 3 µg/ml ab110117 followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

プロトコール

参考文献

ab110117 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10%
Sample
Human Tissue sections (Human brain section)
Specification
Human brain section
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Fixative
Formaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Aug 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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