Anti-DAP Kinase 2 抗体 [EP1633Y] (ab51601)

製品の概要

  • 製品名Anti-DAP Kinase 2 antibody [EP1633Y]
    DAP Kinase 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1633Y] to DAP Kinase 2
  • 特異性ab51601 recognizes DAP Kinase 2.
  • アプリケーション適用あり: WB, IHC-P, Flow Cytmore details
    適用なし: ICC/IF or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues on the N terminal of human DAP Kinase 2.

  • ポジティブ・コントロール
    • WB: Rat spleen lysate IHC-P: Human liver carcinoma.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EP1633Y
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab51601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for ICC/IF or IP.
  • ターゲット情報

    • 機能Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.
    • 組織特異性Ubiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle.
    • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
      Contains 1 protein kinase domain.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DAK2 antibody
      • DAP kinase 2 antibody
      • DAP kinase related protein 1 antibody
      • DAP kinase related protein 1 beta isoform antibody
      • DAP-kinase-related protein 1 antibody
      • DAPK2 antibody
      • DAPK2_HUMAN antibody
      • Death associated protein kinase 2 antibody
      • Death-associated protein kinase 2 antibody
      • DRP 1 antibody
      • DRP-1 antibody
      • DRP1 antibody
      • MGC119312 antibody
      see all

    Anti-DAP Kinase 2 antibody [EP1633Y] 画像

    • Overlay histogram showing HeLa cells stained with ab51601 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51601, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Anti-DAP Kinase 2 antibody [EP1633Y] (ab51601) at 1/2000 dilution + Rat spleen lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 42 kDa
      Observed band size : 42 kDa
    • Immunohistochemical staining of paraffin embedded human liver carcinoma using ab51601 (1/50). Secondary stained using Goat anti-rabbit HRP (1/2000).

    Anti-DAP Kinase 2 antibody [EP1633Y] (ab51601) 使用論文

    This product has been referenced in:
    • Ber Y  et al. DAPK2 is a novel regulator of mTORC1 activity and autophagy. Cell Death Differ 22:465-75 (2015). Read more (PubMed: 25361081) »
    • Shoval Y  et al. New Modularity of DAP-Kinases: Alternative Splicing of the DRP-1 Gene Produces a ZIPk-Like Isoform. PLoS One 6:e17344 (2011). WB ; Human . Read more (PubMed: 21408167) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting us with your questions, and I apologize for any confusion. Since ab51601 is not a purified antibody, we are unable to provide an exact concentration for this product. We recommend using the antibody at the dilutions on the data...

    Read More
    Application Western blot
    Sample African Green Monkey Cell lysate - whole cell (Various cells types (HeLa, 293T, MEFs, COS))
    Loading amount 60 µg
    Specification Various cells types (HeLa, 293T, MEFs, COS)
    Gel Running Conditions Non-reduced Denaturing (10% SDS page gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Mr. Yishay Shoval

    Verified customer

    投稿 Feb 25 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"