Anti-DAGLA 抗体 (ab81984)
Key features and details
- Goat polyclonal to DAGLA
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DAGLA antibody -
製品の詳細
Goat polyclonal to DAGLA -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
C-PAKQDELVISAR
, corresponding to C terminal amino acids 1031-1042 of Human DAGLA. -
ポジティブ・コントロール
- WB: Human liver lysate. IHC-P: Human breast, pancreas, and spleen tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81984の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 115 kDa).
1 hour primary incubation is recommended for this product. |
IHC-P | (3) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 115 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses. -
組織特異性
Highly expressed in brain and pancreas. -
関連疾患
Defects in DAGLA may be a cause of spinocerebellar ataxia type 20 (SCA20) [MIM:608687]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA20 is an autosomal dominant, adult-onset form characterized by dysarthria due to spasmodic dysphonia followed by slowly progressive ataxia. Note=A copy number variation consisting of a 260-kb duplication at chromosome 11q12.2-12.3 is responsible for SCA20. The critical gene within the duplicated segment may be DAGLA. -
配列類似性
Belongs to the AB hydrolase superfamily. Lipase family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 747 Human
- SwissProt: Q9Y4D2 Human
- Unigene: 241564 Human
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別名
- C11orf11 antibody
- Chromosome 11 open reading frame 11 antibody
- DAGL(ALPHA) antibody
see all
画像
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Anti-DAGLA antibody (ab81984) at 0.3 µg/ml + human liver lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 115 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa, 55 kDa. We are unsure as to the identity of these extra bands.Some minor background is detected and is blocked by the immunizing peptide.
Primary incubation for 1 hour. Detected by chemiluminescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab81984 は 7 報の論文で使用されています。
- Mitra S et al. A role for the endocannabinoid enzymes monoacylglycerol and diacylglycerol lipases in cue-induced cocaine craving following prolonged abstinence. Addict Biol 26:e13007 (2021). PubMed: 33496035
- Yoon S et al. cAMP Signaling-Mediated Phosphorylation of Diacylglycerol Lipase α Regulates Interaction With Ankyrin-G and Dendritic Spine Morphology. Biol Psychiatry 90:263-274 (2021). PubMed: 34099188
- Xue SS et al. Repetitive high-frequency transcranial magnetic stimulation reverses depressive-like behaviors and protein expression at hippocampal synapses in chronic unpredictable stress-treated rats by enhancing endocannabinoid signaling. Pharmacol Biochem Behav 184:172738 (2019). PubMed: 31229467
- Smaga I et al. Brain region-dependent changes in the expression of endocannabinoid-metabolizing enzymes in rats following antidepressant drugs. J Physiol Pharmacol 70:N/A (2019). PubMed: 31889042
- Okubo Y et al. Diacylglycerol lipase alpha promotes tumorigenesis in oral cancer by cell-cycle progression. Exp Cell Res 367:112-118 (2018). WB . PubMed: 29614312
- Smaga I et al. Changes in the Brain Endocannabinoid System in Rat Models of Depression. Neurotox Res 31:421-435 (2017). PubMed: 28247204
- Hutch CR et al. An endocannabinoid system is present in the mouse olfactory epithelium but does not modulate olfaction. Neuroscience 300:539-53 (2015). WB ; Mouse . PubMed: 26037800