製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DAGLA
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to internal sequence amino acids 324-354 of Human DAGLA (NP_006124.1).

  • ポジティブ・コントロール
    • Mouse heart tissue lysate, NCI-H292 cell lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: SKNSH.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab106979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 115 kDa.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses.
  • 組織特異性
    Highly expressed in brain and pancreas.
  • 関連疾患
    Defects in DAGLA may be a cause of spinocerebellar ataxia type 20 (SCA20) [MIM:608687]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA20 is an autosomal dominant, adult-onset form characterized by dysarthria due to spasmodic dysphonia followed by slowly progressive ataxia. Note=A copy number variation consisting of a 260-kb duplication at chromosome 11q12.2-12.3 is responsible for SCA20. The critical gene within the duplicated segment may be DAGLA.
  • 配列類似性
    Belongs to the AB hydrolase superfamily. Lipase family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C11orf11 antibody
    • Chromosome 11 open reading frame 11 antibody
    • DAGL(ALPHA) antibody
    • Dagla antibody
    • DAGLALPHA antibody
    • DGL alpha antibody
    • DGL-alpha antibody
    • DGLA_HUMAN antibody
    • Diacylglycerol lipase alpha antibody
    • KIAA0659 antibody
    • Neural stem cell derived dendrite regulator antibody
    • Neural stem cell-derived dendrite regulator antibody
    • NSDDR antibody
    • Sn1-specific diacylglycerol lipase alpha antibody
    see all

画像

  • ICC/IF image of ab106979 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab106979 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-DAGLA antibody (ab106979) at 1/100 dilution + Mouse heart tissue lysate at 35 µg

    Predicted band size : 115 kDa
  • Anti-DAGLA antibody (ab106979) at 1/100 dilution + NCI-H292 cell lysate at 35 µg

    Predicted band size : 115 kDa
  • ab106979 staining mouse cerebellum sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1%BSA for 10 minutes at 21°C, followed by staining with ab106979 at 1/200 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

    DAG L alpha is particularly enriched along the membranes of the secondary dendritic processes of the Purkinje cells, giving a characteristic, striking pattern of positivity.
    The characteristic pattern is seen on the upper image which shows a wt section stained with ab106979 and is not present in the DAG L alpha KO mouse cerebellum section stained with ab106979 (lower left image).

    See Abreview

参考文献

ab106979 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Application
Western blot
Sample
Guinea pig Purified protein (Brain Tissue)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Loading amount
25 µg
Specification
Brain Tissue
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6µg/mL · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Oct 21 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Jul 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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