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Synthetic peptide conjugated to KLH, corresponding to internal sequence amino acids 324-354 of Human DAGLA (NP_006124.1).
Our Abpromise guarantee covers the use of ab106979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 115 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
ICC/IF image of ab106979 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab106979 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab106979 staining mouse cerebellum sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1%BSA for 10 minutes at 21°C, followed by staining with ab106979 at 1/200 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
DAG L alpha is particularly enriched along the membranes of the secondary dendritic processes of the Purkinje cells, giving a characteristic, striking pattern of positivity.
The characteristic pattern is seen on the upper image which shows a wt section stained with ab106979 and is not present in the DAG L alpha KO mouse cerebellum section stained with ab106979 (lower left image).
ab106979 has not yet been referenced specifically in any publications.
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