Anti-DAG1 抗体 (ab110543)

製品の概要

  • 製品名Anti-DAG1 antibody
    DAG1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DAG1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 721-770 (PPSPGSSAAP ATEVPDRDPE KSSEDDVYLH TVIPAVVVAA ILLIAGIIAM), of Mouse DAG1 (NP_034147)

  • ポジティブ・コントロール
    • Mouse heart lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.
    Alpha-dystroglycan is an extracellular peripheral glycoprotein that acts as a receptor for both extracellular matrix proteins containing laminin-G domains, and for certain adenoviruses. Receptor for laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells. Also acts as a receptor for M.leprae in peripheral nerve Schwann cells but only in the presence of the G-domain of LAMA2, and for lymphocytic choriomeningitis virus, Old World Lassa fever virus, and clade C New World arenaviruses.
    Beta-dystroglycan is a transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non-muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity.
  • 組織特異性Expressed in a variety of fetal and adult tissues. In epidermal tissue, located to the basement membrane. Also expressed in keratinocytes and fibroblasts.
  • 関連疾患Defects in DAG1 are the cause of muscular dystrophy-dystroglycanopathy limb-girdle type C7 (MDDGC7) [MIM:613818]. An autosomal recessive muscular dystrophy showing onset in early childhood, and associated with mental retardation without structural brain anomalies. Note=MDDGC7 is caused by DAG1 mutations that interfere with normal post-translational processing, resulting in defective DAG1 glycosylation and impaired interactions with extracellular-matrix components. Other muscular dystrophy-dystroglycanopathies are caused by defects in enzymes involved in protein O-glycosylation.
  • 配列類似性Contains 1 peptidase S72 domain.
  • 翻訳後修飾O- and N-glycosylated. Alpha-dystroglycan is heavily O-glycosylated comprising of up to two thirds of its mass and the carbohydrate composition differs depending on tissue type. Mucin-type O-glycosylation is important for ligand binding activity. O-mannosylation of alpha-DAG1 is found in high abundance in both brain and muscle where the most abundant glycan is Sia-alpha-2-3-Gal-beta-1-4-Glc-NAc-beta-1-2-Man. In muscle, glycosylation on Thr-379 by a phosphorylated O-mannosyl glycan with the structure 2-(N-acetylamido)-2-deoxygalactosyl-beta-1,3-2-(N-acetylamido)-2-deoxyglucosyl-beta-1,4-6-phosphomannose is mediated by like-acetylglucosaminyltransferase (LARGE) protein and is required for laminin binding. O-mannosylation is also required for binding lymphocytic choriomeningitis virus, Old World Lassa fever virus, and clade C New World arenaviruses. The O-glycosyl hexose on Thr-367, Thr-369, Thr-372, Thr-381 and Thr-388 is probably mannose. O-glycosylated in the N-terminal region with a core 1 or possibly core 8 glycan. The beta subunit is N-glycosylated.
    Autolytic cleavage produces the alpha and beta subunits. In cutaneous cells, as well as in certain pathological conditions, shedding of beta-dystroglcan can occur releasing a peptide of about 30 kDa.
    SRC-mediated phosphorylation of the PPXY motif of the beta subunit recruits SH2 domain-containing proteins, but inhibits binding to WWW domain-containing proteins, DMD and UTRN. This phosphorylation also inhibits nuclear entry.
  • 細胞内局在Secreted > extracellular space and Cell membrane. Cytoplasm > cytoskeleton. Nucleus > nucleoplasm. The monomeric form translocates to the nucleus via the action of importins and depends on RAN. Nuclear transport is inhibited by Tyr-892 phosphorylation. In skeletal muscle, this phosphorylated form locates to a vesicular internal membrane compartment. In peripheral nerves, localizes to the Schwann cell membrane. Colocalizes with ERM proteins in Schwann-cell microvilli.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 156DAG antibody
    • A3a antibody
    • Agrin receptor antibody
    • AGRNR antibody
    • Alpha-DG antibody
    • alpha-DG-N antibody
    • Beta-DG antibody
    • Beta-dystroglycan antibody
    • DAG antibody
    • Dag1 antibody
    • DAG1_HUMAN antibody
    • Dystroglycan 1 (dystrophin-associated glycoprotein 1) antibody
    • Dystroglycan antibody
    • Dystroglycan, alpha antibody
    • Dystrophin-associated glycoprotein 1 antibody
    • MDDGC7 antibody
    • MDDGC9 antibody
    • OTTHUMP00000210857 antibody
    • OTTHUMP00000210858 antibody
    see all

Anti-DAG1 antibody 画像

  • Anti-DAG1 antibody (ab110543) at 1 µg/ml + mouse heart lysate at 10 µg

    Predicted band size : 97 kDa

    gel concentration: 12%

     

    Secondary antibody - anti-rabbit HRP (ab6721)

Anti-DAG1 antibody (ab110543) 使用論文

ab110543 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization No
Sample Mouse Cell (mouse embryonic stem cells)
Specification mouse embryonic stem cells
Gating Strategy live cells in fsc vs ssc
Preparation Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 0.2% BSA + 0.1% sodium azide in PBS
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投稿 Apr 30 2014

Application Western blot
Loading amount 1e+006 cells
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Specification mouse embryonic stem cell
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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投稿 Jan 07 2014

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