This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

製品の概要

  • 製品名Anti-Dact2 antibody
    Dact2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Dact2

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Human, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Mouse Dact2.

    (Peptide available as ab42375.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Pancreas (Mouse) Tissue; NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell; Thymus (Mouse) Tissue

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

  • 追加情報WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 82 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors.
    • 配列類似性Belongs to the dapper family.
    • ドメインThe C-terminal PDZ-binding motif may mediate interaction with the PDZ domains of DSH (Dishevelled) family proteins.
    • Information by UniProt
    • 参照データベース
    • 別名
      • bA503C24.7 antibody
      • C6orf116 antibody
      • Chromosome 6 open reading frame 116 antibody
      • Dact2 antibody
      • DACT2_HUMAN antibody
      • Dapper antagonist of beta catenin homolog 2 antibody
      • Dapper antagonist of beta catenin homolog 2 Xenopus laevis antibody
      • Dapper homolog 2 antagonist of beta catenin antibody
      • Dapper homolog 2 antagonist of beta catenin xenopus antibody
      • Dapper homolog 2 antibody
      • DAPPER2 antibody
      • DPR2 antibody
      • FLJ31232 antibody
      • MGC133141 antibody
      • MGC133142 antibody
      • RP11 503C24.7 antibody
      see all

    Anti-Dact2 antibody 画像

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • IHC image of Dact2 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42099, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    • All lanes : Anti-Dact2 antibody (ab42099) at 1 µg/ml

      Lane 1 : Mouse pancreas tissue lysate - total protein (ab29363)
      Lane 2 : NIH 3T3 whole cell lysate (ab7179)
      Lane 3 : Mouse thymus tissue lysate - total protein (ab29285)

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 82 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Dact2 antibody (ab42099) at 1 µg/ml

      Lane 1 : Kidney (Mouse) Tissue Lysate
      Lane 2 : Brain (Mouse) Tissue Lysate
      Lane 3 : Human kidney tissue lysate - total protein (ab30203)
      Lane 4 : Human brain tissue lysate - total protein (ab29466)
      Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 6 : SK N SH (Human neuroblastoma) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 82 kDa
      Observed band size : 115 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 2 minutes

    Anti-Dact2 antibody (ab42099) 使用論文

    ab42099 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab42099.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"