製品の概要

  • 製品名Anti-DACH1 antibody [EPR2841Y]
    DACH1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2841Y] to DACH1
  • アプリケーション適用あり: WB, IP, Flow Cytmore details
    適用なし: ICC/IF or IHC-P
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C terminus of human DACH1

  • ポジティブ・コントロール
    • MCF-7 and HeLa cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
Flow Cyt
  • 追加情報Flow Cyt: 1/120.
    IP: 1/40.
    WB: 1/500 - 1/1000.Predicted molecular weight: 79 kDa.

    Is unsuitable for ICC/IF or IHC-P.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter (By similarity). Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain.
    • 組織特異性Widely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart. Isoform 4 is found in spleen.
    • 配列類似性Belongs to the DACH/dachshund family.
    • ドメインThe DACHbox-N/DD1 domain forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DACH antibody
      • Dach1 antibody
      • DACH1_HUMAN antibody
      • dachshund homolog 1 (Drosophila) antibody
      • Dachshund homolog 1 antibody
      • Dachsund homolog 1 antibody
      • FLJ10138 antibody
      • OTTHUMP00000018494 antibody
      see all

    Anti-DACH1 antibody [EPR2841Y] 画像

    • All lanes : Anti-DACH1 antibody [EPR2841Y] (ab77234) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : Hela cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 79 kDa
      Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    • Overlay histogram showing HeLa cells stained with ab77234 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77234, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Anti-DACH1 antibody [EPR2841Y] (ab77234) 使用論文

    This product has been referenced in:
    • Lutay N  et al. Bacterial control of host gene expression through RNA polymerase II. J Clin Invest 123:2366-79 (2013). WB ; Human . Read more (PubMed: 23728172) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution - Vector h3300
    Sample Human Tissue sections (adrenal tumour)
    Specification adrenal tumour
    Permeabilization No
    Fixative Methanol
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    投稿 Sep 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"