製品の概要

  • 製品名Anti-DAB2IP antibody
  • 製品の詳細
    Rabbit polyclonal to DAB2IP
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 850 and 900 of human DAB2IP (CAQ10385.1).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab87811 was affinity purified using an epitope specific to DAB2IP immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab87811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 132 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性DAB2IP is a Ras GTPase-activating protein (GAP) that acts as a tumor suppressor gene and is inactivated by methylation in prostate and breast cancers. It also interacts with MAP3K5 to disrupt the association of MAP3K5 with the inhibitory 14-3-3 complex.
  • 細胞内局在Cytoplasmic
  • 参照データベース
  • 別名
    • AF9Q34 antibody
    • AIP1 antibody
    • ASK interacting protein antibody
    • ASK interacting protein 1 antibody
    • ASK1 interacting protein 1 antibody
    • DAB2 interacting protein antibody
    • DAB2 interaction protein antibody
    • DAB2P antibody
    • DIP1/2 antibody
    • Disabled homolog 2 interacting protein antibody
    • DOC 2/DAB2 interactive protein antibody
    • FLJ39072 antibody
    • KIAA1743 antibody
    • nGAP like protein antibody
    see all

Anti-DAB2IP antibody 画像

  • All lanes : Anti-DAB2IP antibody (ab87811) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 132 kDa
    Observed band size : 132 kDa


    Exposure time : 3 minutes
  • Detection of DAB2IP in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87811 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
    Detection: Chemiluminescence with an exposure time of 3 seconds.
  • IHC image of ab87811 staining in normal human breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87811, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-DAB2IP antibody (ab87811) 使用論文

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry the anti-DAB2IP antibody ab87811 did not perform as stated on the datasheet and for th...

Read More

Thank you for confirming these details. The details you have kindly provided will provide us with vital information for our monitoring of product quality. Although it is not necessary to add further loading buffer to the lysates you purchased from us, ...

Read More

Thank you for taking the time tocontact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this Anti-DAB2IP antibody. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"