The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use at an assay dependent concentration.
Use a concentration of 2 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 83, 58.8 kDa).
DAB2 is a component of the CSF1 signal transduction pathway. DAB2 mRNA is expressed in normal ovarian epithelial cells but is down regulated or absent from ovarian carcinoma cell lines. The down regulation of DAB2 may play an important role in ovarian carcinogenesis. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2 like domains, alpha and beta).
Cytoplasmic vesicle; clathrin coated vesicle; clathrin coated vesicle membrane. Cell membrane; coated pit; clathrin coated pit.
Note: Colocalizes with large insert containing isoforms of MYO6 at clathrin coated pits/vesicles.
This antibody was raised against an immunogen that is predicted to cross react with both isoforms 1 and 2 of Human DAB2 . We believe that the band observed is isoform 1 due to its higher molecular weight of 102 kDa. While this differs to its predicted molecular weight of 83 kDa (Swiss-Prot data) this migration has been observed in the literature and may be due to modifications at potential phosphorylation sites. (Am J Pathol. 2006 Jul;169(1):258-67. PMID: 16816378).
ICC/IF image of ab33441 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab33441 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.