製品の概要

  • 製品名Anti-DAB2 antibody
    DAB2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DAB2
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Zebrafish, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human DAB2.

    (Peptide available as ab33440.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Testis Tissue Lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab33441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 83, 58.8 kDa).

ターゲット情報

  • 関連性DAB2 is a component of the CSF1 signal transduction pathway. DAB2 mRNA is expressed in normal ovarian epithelial cells but is down regulated or absent from ovarian carcinoma cell lines. The down regulation of DAB2 may play an important role in ovarian carcinogenesis. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2 like domains, alpha and beta).
  • 細胞内局在Cytoplasmic vesicle; clathrin coated vesicle; clathrin coated vesicle membrane. Cell membrane; coated pit; clathrin coated pit. Note: Colocalizes with large insert containing isoforms of MYO6 at clathrin coated pits/vesicles.
  • 参照データベース
  • 別名
    • DAB 2 antibody
    • Dab, mitogen-responsive phosphoprotein, homolog 2 (Drosophila) antibody
    • DAB2 antibody
    • Differentially expressed in ovarian cancer 2 antibody
    • Differentially expressed protein 2 antibody
    • Disabled (Drosophila) homolog 2 (mitogen responsive phosphoprotein) antibody
    • Disabled homolog 2 antibody
    • Disabled homolog 2 mitogen responsive phosphoprotein (Drosophila) antibody
    • Disabled homolog 2 mitogen responsive phosphoprotein antibody
    • Disabled, Drosophila, homolog of, 2 antibody
    • DOC 2 antibody
    • DOC2 antibody
    • FLJ26626 antibody
    • Mitogen responsive phosphoprotein antibody
    see all

Anti-DAB2 antibody 画像

  • Anti-DAB2 antibody (ab33441) at 2 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 83, 58.8 kDa
    Observed band size : 102 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab33441 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab33441 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DAB2 antibody (ab33441) 使用論文

This product has been referenced in:
  • Abrami L  et al. Endocytosis of the anthrax toxin is mediated by clathrin, actin and unconventional adaptors. PLoS Pathog 6:e1000792 (2010). WB ; Human . Read more (PubMed: 20221438) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (placenta)
Specification placenta
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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投稿 May 25 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"